IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v467y2010i7315d10.1038_nature09411.html
   My bibliography  Save this article

Two enzymes bound to one transfer RNA assume alternative conformations for consecutive reactions

Author

Listed:
  • Takuhiro Ito

    (Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
    Laboratory of Structural Biology, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
    RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama City, Kanagawa 230-0045, Japan)

  • Shigeyuki Yokoyama

    (Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
    Laboratory of Structural Biology, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
    RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama City, Kanagawa 230-0045, Japan)

Abstract

Enzymes sharing space on tRNA Synthesis of Glu-tRNA(Glu) and Gln-tRNA(Gln) begins with a common reaction, in which the glutamyl-tRNA synthetase (GluRS) glutamylates both tRNA(Glu) and tRNA(Gln). The Glu-tRNA(Gln) is then specifically converted to Gln-tRNA(Gln) by an amidotransferase. Takuhiro Ito and Shigeyuki Yokoyama have now solved the structure of a bacterial macromolecular 'glutamine transamidosome' complex containing tRNA(Gln), GluRS and the trimeric amidotransferase GatCAB. The structure explains the hand-off mechanism between the two enzymes, in which only one can assume a productive state at any time, and suggests that there is an intermediary state in which neither enzyme is productive.

Suggested Citation

  • Takuhiro Ito & Shigeyuki Yokoyama, 2010. "Two enzymes bound to one transfer RNA assume alternative conformations for consecutive reactions," Nature, Nature, vol. 467(7315), pages 612-616, September.
    • Handle: RePEc:nat:nature:v:467:y:2010:i:7315:d:10.1038_nature09411
    DOI: 10.1038/nature09411
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/nature09411
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/nature09411?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Satoshi Kimura & Veerasak Srisuknimit & Kacie L. McCarty & Peter C. Dedon & Philip J. Kranzusch & Matthew K. Waldor, 2022. "Sequential action of a tRNA base editor in conversion of cytidine to pseudouridine," Nature Communications, Nature, vol. 13(1), pages 1-15, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:467:y:2010:i:7315:d:10.1038_nature09411. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.