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Mechanism of the ATP-dependent DNA end-resection machinery from Saccharomyces cerevisiae

Author

Listed:
  • Hengyao Niu

    (Yale University School of Medicine)

  • Woo-Hyun Chung

    (Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA)

  • Zhu Zhu

    (Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA)

  • Youngho Kwon

    (Yale University School of Medicine)

  • Weixing Zhao

    (Yale University School of Medicine)

  • Peter Chi

    (Yale University School of Medicine)

  • Rohit Prakash

    (Yale University School of Medicine)

  • Changhyun Seong

    (Yale University School of Medicine)

  • Dongqing Liu

    (Yale University School of Medicine)

  • Lucy Lu

    (Yale University School of Medicine)

  • Grzegorz Ira

    (Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA)

  • Patrick Sung

    (Yale University School of Medicine)

Abstract

Priming DNA for repair When DNA damage introduces double-strand breaks, the ends formed must undergo processing to prepare them for repair. In related studies by the Sung and Kowalczykowski laboratories, this processing reaction has been replicated in vitro using yeast proteins. Processing minimally requires the activities of a helicase, a nuclease and a single-strand binding protein, although the reaction is enhanced by further addition of three factors that help target the core complex and enhance the unwinding activity.

Suggested Citation

  • Hengyao Niu & Woo-Hyun Chung & Zhu Zhu & Youngho Kwon & Weixing Zhao & Peter Chi & Rohit Prakash & Changhyun Seong & Dongqing Liu & Lucy Lu & Grzegorz Ira & Patrick Sung, 2010. "Mechanism of the ATP-dependent DNA end-resection machinery from Saccharomyces cerevisiae," Nature, Nature, vol. 467(7311), pages 108-111, September.
    • Handle: RePEc:nat:nature:v:467:y:2010:i:7311:d:10.1038_nature09318
    DOI: 10.1038/nature09318
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    Citations

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    Cited by:

    1. Lorenzo Galanti & Martina Peritore & Robert Gnügge & Elda Cannavo & Johannes Heipke & Maria Dilia Palumbieri & Barbara Steigenberger & Lorraine S. Symington & Petr Cejka & Boris Pfander, 2024. "Dbf4-dependent kinase promotes cell cycle controlled resection of DNA double-strand breaks and repair by homologous recombination," Nature Communications, Nature, vol. 15(1), pages 1-19, December.
    2. Priya Kapoor-Vazirani & Sandip K. Rath & Xu Liu & Zhen Shu & Nicole E. Bowen & Yitong Chen & Ramona Haji-Seyed-Javadi & Waaqo Daddacha & Elizabeth V. Minten & Diana Danelia & Daniela Farchi & Duc M. D, 2022. "SAMHD1 deacetylation by SIRT1 promotes DNA end resection by facilitating DNA binding at double-strand breaks," Nature Communications, Nature, vol. 13(1), pages 1-18, December.
    3. Ananya Acharya & Kristina Kasaciunaite & Martin Göse & Vera Kissling & Raphaël Guérois & Ralf Seidel & Petr Cejka, 2021. "Distinct RPA domains promote recruitment and the helicase-nuclease activities of Dna2," Nature Communications, Nature, vol. 12(1), pages 1-15, December.
    4. Chaoyou Xue & Sameer J. Salunkhe & Nozomi Tomimatsu & Ajinkya S. Kawale & Youngho Kwon & Sandeep Burma & Patrick Sung & Eric C. Greene, 2022. "Bloom helicase mediates formation of large single–stranded DNA loops during DNA end processing," Nature Communications, Nature, vol. 13(1), pages 1-16, December.

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