IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v464y2010i7293d10.1038_nature08948.html
   My bibliography  Save this article

Tracking G-protein-coupled receptor activation using genetically encoded infrared probes

Author

Listed:
  • Shixin Ye

    (Laboratory of Molecular Biology and Biochemistry, The Rockefeller University, 1230 York Avenue, New York, New York 10065, USA)

  • Ekaterina Zaitseva

    (Biophysics Section, Institute of Molecular Medicine and Cell Research, University of Freiburg, Hermann Herder Str. 9, D-79104 Freiburg, Germany)

  • Gianluigi Caltabiano

    (Laboratori de Medicina Computacional, Unitat de Bioestadística, Universitat Autònoma de Barcelona, 08193 Bellaterra, Catalunya, Spain)

  • Gebhard F. X. Schertler

    (MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Laboratory of Biomolecular Research, Paul Scherrer Institut)

  • Thomas P. Sakmar

    (Laboratory of Molecular Biology and Biochemistry, The Rockefeller University, 1230 York Avenue, New York, New York 10065, USA)

  • Xavier Deupi

    (Laboratori de Medicina Computacional, Unitat de Bioestadística, Universitat Autònoma de Barcelona, 08193 Bellaterra, Catalunya, Spain)

  • Reiner Vogel

    (Biophysics Section, Institute of Molecular Medicine and Cell Research, University of Freiburg, Hermann Herder Str. 9, D-79104 Freiburg, Germany)

Abstract

Rhodopsin activation tracked The G-protein-coupled receptor (GPCR) rhodopsin is responsible for dim-light vision. Incoming light isomerizes the protein's retinal chromophore and triggers concerted movements of several transmembrane helices. Here, site-directed non-natural amino acid mutagenesis is used to engineer mutant rhodopsins containing a p-azido-L-phenylalanine residue at selected sites. The azido probe's vibrational signatures can then be monitored using infrared spectroscopy, revealing changes in the electrostatic environment as rhodopsin proceeds along its activation pathway. The technique reveals early conformational changes in the protein that precede the well known larger movements of the transmembrane helices.

Suggested Citation

  • Shixin Ye & Ekaterina Zaitseva & Gianluigi Caltabiano & Gebhard F. X. Schertler & Thomas P. Sakmar & Xavier Deupi & Reiner Vogel, 2010. "Tracking G-protein-coupled receptor activation using genetically encoded infrared probes," Nature, Nature, vol. 464(7293), pages 1386-1389, April.
    • Handle: RePEc:nat:nature:v:464:y:2010:i:7293:d:10.1038_nature08948
    DOI: 10.1038/nature08948
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/nature08948
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/nature08948?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Arum Wu & David Salom & John D. Hong & Aleksander Tworak & Kohei Watanabe & Els Pardon & Jan Steyaert & Hideki Kandori & Kota Katayama & Philip D. Kiser & Krzysztof Palczewski, 2023. "Structural basis for the allosteric modulation of rhodopsin by nanobody binding to its extracellular domain," Nature Communications, Nature, vol. 14(1), pages 1-17, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:464:y:2010:i:7293:d:10.1038_nature08948. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.