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Retroviral intasome assembly and inhibition of DNA strand transfer

Author

Listed:
  • Stephen Hare

    (Imperial College London, St-Mary’s Campus, Norfolk Place, London W2 1PG, UK)

  • Saumya Shree Gupta

    (Imperial College London, St-Mary’s Campus, Norfolk Place, London W2 1PG, UK
    Present addresses: Hannover Biomedical Research School, D-30625 Hannover, Germany (S.S.G.); School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland 4072, Australia (E.V.).)

  • Eugene Valkov

    (Imperial College London, St-Mary’s Campus, Norfolk Place, London W2 1PG, UK
    Present addresses: Hannover Biomedical Research School, D-30625 Hannover, Germany (S.S.G.); School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland 4072, Australia (E.V.).)

  • Alan Engelman

    (Dana-Farber Cancer Institute, 44 Binney Street, Boston, Massachusetts 02115, USA)

  • Peter Cherepanov

    (Imperial College London, St-Mary’s Campus, Norfolk Place, London W2 1PG, UK)

Abstract

Integrase is an essential retroviral enzyme that binds both termini of linear viral DNA and inserts them into a host cell chromosome. The structure of full-length retroviral integrase, either separately or in complex with DNA, has been lacking. Furthermore, although clinically useful inhibitors of HIV integrase have been developed, their mechanism of action remains speculative. Here we present a crystal structure of full-length integrase from the prototype foamy virus in complex with its cognate DNA. The structure shows the organization of the retroviral intasome comprising an integrase tetramer tightly associated with a pair of viral DNA ends. All three canonical integrase structural domains are involved in extensive protein–DNA and protein–protein interactions. The binding of strand-transfer inhibitors displaces the reactive viral DNA end from the active site, disarming the viral nucleoprotein complex. Our findings define the structural basis of retroviral DNA integration, and will allow modelling of the HIV-1 intasome to aid in the development of antiretroviral drugs.

Suggested Citation

  • Stephen Hare & Saumya Shree Gupta & Eugene Valkov & Alan Engelman & Peter Cherepanov, 2010. "Retroviral intasome assembly and inhibition of DNA strand transfer," Nature, Nature, vol. 464(7286), pages 232-236, March.
    • Handle: RePEc:nat:nature:v:464:y:2010:i:7286:d:10.1038_nature08784
    DOI: 10.1038/nature08784
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    Cited by:

    1. Mercedes Spínola-Amilibia & Lidia Araújo-Bazán & Álvaro Gándara & James M. Berger & Ernesto Arias-Palomo, 2023. "IS21 family transposase cleaved donor complex traps two right-handed superhelical crossings," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    2. Gayan Senavirathne & James London & Anne Gardner & Richard Fishel & Kristine E. Yoder, 2023. "DNA strand breaks and gaps target retroviral intasome binding and integration," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    3. Allison Ballandras-Colas & Vidya Chivukula & Dominika T. Gruszka & Zelin Shan & Parmit K. Singh & Valerie E. Pye & Rebecca K. McLean & Gregory J. Bedwell & Wen Li & Andrea Nans & Nicola J. Cook & Hind, 2022. "Multivalent interactions essential for lentiviral integrase function," Nature Communications, Nature, vol. 13(1), pages 1-16, December.

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