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SSB protein diffusion on single-stranded DNA stimulates RecA filament formation

Author

Listed:
  • Rahul Roy

    (Center for Biophysics and Computational Biology,
    University of Illinois, Urbana-Champaign, Illinois 61801, USA
    Present address: Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA.)

  • Alexander G. Kozlov

    (Washington University School of Medicine, St Louis, Missouri 63110, USA)

  • Timothy M. Lohman

    (Washington University School of Medicine, St Louis, Missouri 63110, USA)

  • Taekjip Ha

    (Center for Biophysics and Computational Biology,
    University of Illinois, Urbana-Champaign, Illinois 61801, USA
    Howard Hughes Medical Institute, Urbana, Illinois 61801, USA)

Abstract

Single-stranded DNA generated in the cell during DNA metabolism is stabilized and protected by binding of ssDNA-binding (SSB) proteins. Escherichia coli SSB, a representative homotetrameric SSB, binds to ssDNA by wrapping the DNA using its four subunits. However, such a tightly wrapped, high-affinity protein–DNA complex still needs to be removed or repositioned quickly for unhindered action of other proteins. Here we show, using single-molecule two- and three-colour fluorescence resonance energy transfer, that tetrameric SSB can spontaneously migrate along ssDNA. Diffusional migration of SSB helps in the local displacement of SSB by an elongating RecA filament. SSB diffusion also melts short DNA hairpins transiently and stimulates RecA filament elongation on DNA with secondary structure. This observation of diffusional movement of a protein on ssDNA introduces a new model for how an SSB protein can be redistributed, while remaining tightly bound to ssDNA during recombination and repair processes.

Suggested Citation

  • Rahul Roy & Alexander G. Kozlov & Timothy M. Lohman & Taekjip Ha, 2009. "SSB protein diffusion on single-stranded DNA stimulates RecA filament formation," Nature, Nature, vol. 461(7267), pages 1092-1097, October.
    • Handle: RePEc:nat:nature:v:461:y:2009:i:7267:d:10.1038_nature08442
    DOI: 10.1038/nature08442
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    Cited by:

    1. Andreas Hartmann & Koushik Sreenivasa & Mathias Schenkel & Neharika Chamachi & Philipp Schake & Georg Krainer & Michael Schlierf, 2023. "An automated single-molecule FRET platform for high-content, multiwell plate screening of biomolecular conformations and dynamics," Nature Communications, Nature, vol. 14(1), pages 1-12, December.

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