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Coordination of Rho GTPase activities during cell protrusion

Author

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  • Matthias Machacek

    (The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, California 92037, USA
    Present addresses: Novartis Pharma AG, Lichtstrasse 35, CH-4056 Basel, Switzerland (M.M.); Department of Anatomy and Structural Biology and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine of Yeshiva University, 1300 Morris Park Ave, Bronx, New York 10461, USA (L.H.); Department of Biomedicine, University of Basel, Mattenstrasse 28, CH-4058 Basel, Switzerland (O.P.).)

  • Louis Hodgson

    (Medicinal Chemistry and Lineberger Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Present addresses: Novartis Pharma AG, Lichtstrasse 35, CH-4056 Basel, Switzerland (M.M.); Department of Anatomy and Structural Biology and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine of Yeshiva University, 1300 Morris Park Ave, Bronx, New York 10461, USA (L.H.); Department of Biomedicine, University of Basel, Mattenstrasse 28, CH-4058 Basel, Switzerland (O.P.).)

  • Christopher Welch

    (Medicinal Chemistry and Lineberger Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA)

  • Hunter Elliott

    (The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, California 92037, USA)

  • Olivier Pertz

    (The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, California 92037, USA
    Present addresses: Novartis Pharma AG, Lichtstrasse 35, CH-4056 Basel, Switzerland (M.M.); Department of Anatomy and Structural Biology and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine of Yeshiva University, 1300 Morris Park Ave, Bronx, New York 10461, USA (L.H.); Department of Biomedicine, University of Basel, Mattenstrasse 28, CH-4058 Basel, Switzerland (O.P.).)

  • Perihan Nalbant

    (Center for Medical Biotechnology, University of Duisburg-Essen)

  • Amy Abell

    (Medicinal Chemistry and Lineberger Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA)

  • Gary L. Johnson

    (Medicinal Chemistry and Lineberger Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA)

  • Klaus M. Hahn

    (Medicinal Chemistry and Lineberger Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA)

  • Gaudenz Danuser

    (The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, California 92037, USA)

Abstract

Rho GTPases during cell protrusion The Rho GTPase family acts in concert to regulate cyoskeletal dynamics during processes such as cell motility. In this study, Danuser and colleagues study the coordination of RhoA, Rac1 and Cdc42 during cell migration by simultaneously visualizing two molecules using complementary biosensor designs, and by computationally defining the relationships between individual molecules visualized in separate cells. The latter approach demonstrates that different biosensors, imaged separately, can be freely combined to produce maps of relative signalling activities with seconds and single-micron resolution. These technologies pave the way to defining the dynamics of many proteins in large signal transduction networks.

Suggested Citation

  • Matthias Machacek & Louis Hodgson & Christopher Welch & Hunter Elliott & Olivier Pertz & Perihan Nalbant & Amy Abell & Gary L. Johnson & Klaus M. Hahn & Gaudenz Danuser, 2009. "Coordination of Rho GTPase activities during cell protrusion," Nature, Nature, vol. 461(7260), pages 99-103, September.
    • Handle: RePEc:nat:nature:v:461:y:2009:i:7260:d:10.1038_nature08242
    DOI: 10.1038/nature08242
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    Cited by:

    1. Despoina Kyriazi & Lea Voth & Almke Bader & Wiebke Ewert & Juliane Gerlach & Kerstin Elfrink & Peter Franz & Mariana I. Tsap & Bastian Schirmer & Julia Damiano-Guercio & Falk K. Hartmann & Masina Plen, 2024. "An allosteric inhibitor of RhoGAP class-IX myosins suppresses the metastatic features of cancer cells," Nature Communications, Nature, vol. 15(1), pages 1-25, December.
    2. Suchet Nanda & Abram Calderon & Arya Sachan & Thanh-Thuy Duong & Johannes Koch & Xiaoyi Xin & Djamschid Solouk-Stahlberg & Yao-Wen Wu & Perihan Nalbant & Leif Dehmelt, 2023. "Rho GTPase activity crosstalk mediated by Arhgef11 and Arhgef12 coordinates cell protrusion-retraction cycles," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    3. Shu Fan Zhou & Singaram Gopalakrishnan & Yuan Hao Xu & Jie Yang & Yun Wah Lam & Stella W Pang, 2016. "A Unidirectional Cell Switching Gate by Engineering Grating Length and Bending Angle," PLOS ONE, Public Library of Science, vol. 11(1), pages 1-18, January.
    4. Michelle Maurin & Mohammadreza Ranjouri & Cristina Megino-Luque & Justin Y. Newberg & Dongliang Du & Katelyn Martin & Robert E. Miner & Mollie S. Prater & Dave Keng Boon Wee & Barbara Centeno & Shondr, 2023. "RBFOX2 deregulation promotes pancreatic cancer progression and metastasis through alternative splicing," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    5. Vinal V Lakhani & Elizabeth Hinde & Enrico Gratton & Timothy C Elston, 2015. "Spatio-Temporal Regulation of Rac1 Mobility by Actin Islands," PLOS ONE, Public Library of Science, vol. 10(11), pages 1-14, November.
    6. Anna Bianchi-Smiraglia & David W. Wolff & Daniel J. Marston & Zhiyong Deng & Zhannan Han & Sudha Moparthy & Rebecca M. Wombacher & Ashley L. Mussell & Shichen Shen & Jialin Chen & Dong-Hyun Yun & Ande, 2021. "Regulation of local GTP availability controls RAC1 activity and cell invasion," Nature Communications, Nature, vol. 12(1), pages 1-15, December.

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