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The active form of DNA polymerase V is UmuD′2C–RecA–ATP

Author

Listed:
  • Qingfei Jiang

    (University of Southern California, University Park, Los Angeles, California 90089-2910, USA)

  • Kiyonobu Karata

    (Laboratory of Genomic Integrity, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892-3371, USA)

  • Roger Woodgate

    (Laboratory of Genomic Integrity, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892-3371, USA)

  • Michael M. Cox

    (University of Wisconsin-Madison, Madison, Wisconsin 53706, USA)

  • Myron F. Goodman

    (University of Southern California, University Park, Los Angeles, California 90089-2910, USA)

Abstract

DNA-damage-induced SOS mutations arise when Escherichia coli DNA polymerase (pol) V, activated by a RecA nucleoprotein filament (RecA*), catalyses translesion DNA synthesis. Here we address two longstanding enigmatic aspects of SOS mutagenesis, the molecular composition of mutagenically active pol V and the role of RecA*. We show that RecA* transfers a single RecA–ATP stoichiometrically from its DNA 3′-end to free pol V (UmuD′2C) to form an active mutasome (pol V Mut) with the composition UmuD′2C–RecA–ATP. Pol V Mut catalyses TLS in the absence of RecA* and deactivates rapidly upon dissociation from DNA. Deactivation occurs more slowly in the absence of DNA synthesis, while retaining RecA–ATP in the complex. Reactivation of pol V Mut is triggered by replacement of RecA–ATP from RecA*. Thus, the principal role of RecA* in SOS mutagenesis is to transfer RecA–ATP to pol V, and thus generate active mutasomal complex for translesion synthesis.

Suggested Citation

  • Qingfei Jiang & Kiyonobu Karata & Roger Woodgate & Michael M. Cox & Myron F. Goodman, 2009. "The active form of DNA polymerase V is UmuD′2C–RecA–ATP," Nature, Nature, vol. 460(7253), pages 359-363, July.
    • Handle: RePEc:nat:nature:v:460:y:2009:i:7253:d:10.1038_nature08178
    DOI: 10.1038/nature08178
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    Cited by:

    1. Andrew Robinson & John P McDonald & Victor E A Caldas & Meghna Patel & Elizabeth A Wood & Christiaan M Punter & Harshad Ghodke & Michael M Cox & Roger Woodgate & Myron F Goodman & Antoine M van Oijen, 2015. "Regulation of Mutagenic DNA Polymerase V Activation in Space and Time," PLOS Genetics, Public Library of Science, vol. 11(8), pages 1-30, August.

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