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Generation of transgenic non-human primates with germline transmission

Author

Listed:
  • Erika Sasaki

    (Central Institute for Experimental Animals, 1430 Nogawa, Miyamae-ku, Kawasaki, Kanagawa 216-0001, Japan)

  • Hiroshi Suemizu

    (Central Institute for Experimental Animals, 1430 Nogawa, Miyamae-ku, Kawasaki, Kanagawa 216-0001, Japan)

  • Akiko Shimada

    (Central Institute for Experimental Animals, 1430 Nogawa, Miyamae-ku, Kawasaki, Kanagawa 216-0001, Japan)

  • Kisaburo Hanazawa

    (Juntendo University Nerima Hospital 3-1-10 Takanodai, Nerima-ku, Tokyo 177-8521, Japan)

  • Ryo Oiwa

    (Central Institute for Experimental Animals, 1430 Nogawa, Miyamae-ku, Kawasaki, Kanagawa 216-0001, Japan)

  • Michiko Kamioka

    (Central Institute for Experimental Animals, 1430 Nogawa, Miyamae-ku, Kawasaki, Kanagawa 216-0001, Japan)

  • Ikuo Tomioka

    (Central Institute for Experimental Animals, 1430 Nogawa, Miyamae-ku, Kawasaki, Kanagawa 216-0001, Japan
    Center for Integrated Medical Research,)

  • Yusuke Sotomaru

    (Natural Science Centre for Basic Research and Development, Hiroshima University 1-2-3, Kasumi, Minami-ku, Hiroshima 734-8551, Japan)

  • Reiko Hirakawa

    (Central Institute for Experimental Animals, 1430 Nogawa, Miyamae-ku, Kawasaki, Kanagawa 216-0001, Japan
    Center for Integrated Medical Research,)

  • Tomoo Eto

    (Central Institute for Experimental Animals, 1430 Nogawa, Miyamae-ku, Kawasaki, Kanagawa 216-0001, Japan)

  • Seiji Shiozawa

    (Central Institute for Experimental Animals, 1430 Nogawa, Miyamae-ku, Kawasaki, Kanagawa 216-0001, Japan
    Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan)

  • Takuji Maeda

    (Central Institute for Experimental Animals, 1430 Nogawa, Miyamae-ku, Kawasaki, Kanagawa 216-0001, Japan
    Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan)

  • Mamoru Ito

    (Central Institute for Experimental Animals, 1430 Nogawa, Miyamae-ku, Kawasaki, Kanagawa 216-0001, Japan)

  • Ryoji Ito

    (Central Institute for Experimental Animals, 1430 Nogawa, Miyamae-ku, Kawasaki, Kanagawa 216-0001, Japan)

  • Chika Kito

    (Central Institute for Experimental Animals, 1430 Nogawa, Miyamae-ku, Kawasaki, Kanagawa 216-0001, Japan)

  • Chie Yagihashi

    (Central Institute for Experimental Animals, 1430 Nogawa, Miyamae-ku, Kawasaki, Kanagawa 216-0001, Japan)

  • Kenji Kawai

    (Central Institute for Experimental Animals, 1430 Nogawa, Miyamae-ku, Kawasaki, Kanagawa 216-0001, Japan)

  • Hiroyuki Miyoshi

    (Subteam for Manipulation of Cell Fate, RIKEN BioResource Centre, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan)

  • Yoshikuni Tanioka

    (Central Institute for Experimental Animals, 1430 Nogawa, Miyamae-ku, Kawasaki, Kanagawa 216-0001, Japan)

  • Norikazu Tamaoki

    (Central Institute for Experimental Animals, 1430 Nogawa, Miyamae-ku, Kawasaki, Kanagawa 216-0001, Japan)

  • Sonoko Habu

    (Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa 259-1193, Japan)

  • Hideyuki Okano

    (Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan)

  • Tatsuji Nomura

    (Central Institute for Experimental Animals, 1430 Nogawa, Miyamae-ku, Kawasaki, Kanagawa 216-0001, Japan)

Abstract

The common marmoset (Callithrix jacchus) is increasingly attractive for use as a non-human primate animal model in biomedical research. It has a relatively high reproduction rate for a primate, making it potentially suitable for transgenic modification. Although several attempts have been made to produce non-human transgenic primates, transgene expression in the somatic tissues of live infants has not been demonstrated by objective analyses such as polymerase chain reaction with reverse transcription or western blots. Here we show that the injection of a self-inactivating lentiviral vector in sucrose solution into marmoset embryos results in transgenic common marmosets that expressed the transgene in several organs. Notably, we achieved germline transmission of the transgene, and the transgenic offspring developed normally. The successful creation of transgenic marmosets provides a new animal model for human disease that has the great advantage of a close genetic relationship with humans. This model will be valuable to many fields of biomedical research.

Suggested Citation

  • Erika Sasaki & Hiroshi Suemizu & Akiko Shimada & Kisaburo Hanazawa & Ryo Oiwa & Michiko Kamioka & Ikuo Tomioka & Yusuke Sotomaru & Reiko Hirakawa & Tomoo Eto & Seiji Shiozawa & Takuji Maeda & Mamoru I, 2009. "Generation of transgenic non-human primates with germline transmission," Nature, Nature, vol. 459(7246), pages 523-527, May.
    • Handle: RePEc:nat:nature:v:459:y:2009:i:7246:d:10.1038_nature08090
    DOI: 10.1038/nature08090
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    Cited by:

    1. Kyle M. Gilbert & Justine C. Cléry & Joseph S. Gati & Yuki Hori & Kevin D. Johnston & Alexander Mashkovtsev & Janahan Selvanayagam & Peter Zeman & Ravi S. Menon & David J. Schaeffer & Stefan Everling, 2021. "Simultaneous functional MRI of two awake marmosets," Nature Communications, Nature, vol. 12(1), pages 1-11, December.
    2. Patrick Jendritza & Frederike J. Klein & Pascal Fries, 2023. "Multi-area recordings and optogenetics in the awake, behaving marmoset," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    3. A. Calapai & J. Cabrera-Moreno & T. Moser & M. Jeschke, 2022. "Flexible auditory training, psychophysics, and enrichment of common marmosets with an automated, touchscreen-based system," Nature Communications, Nature, vol. 13(1), pages 1-16, December.

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