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Precise genome modification in the crop species Zea mays using zinc-finger nucleases

Author

Listed:
  • Vipula K. Shukla

    (Dow AgroSciences, 9330 Zionsville Road, Indianapolis, Indiana 46268, USA)

  • Yannick Doyon

    (Sangamo BioSciences, Point Richmond Tech Center, 501 Canal Boulevard, Suite A100, Richmond, California 94804, USA)

  • Jeffrey C. Miller

    (Sangamo BioSciences, Point Richmond Tech Center, 501 Canal Boulevard, Suite A100, Richmond, California 94804, USA)

  • Russell C. DeKelver

    (Sangamo BioSciences, Point Richmond Tech Center, 501 Canal Boulevard, Suite A100, Richmond, California 94804, USA)

  • Erica A. Moehle

    (Sangamo BioSciences, Point Richmond Tech Center, 501 Canal Boulevard, Suite A100, Richmond, California 94804, USA)

  • Sarah E. Worden

    (Dow AgroSciences, 9330 Zionsville Road, Indianapolis, Indiana 46268, USA)

  • Jon C. Mitchell

    (Dow AgroSciences, 9330 Zionsville Road, Indianapolis, Indiana 46268, USA)

  • Nicole L. Arnold

    (Dow AgroSciences, 9330 Zionsville Road, Indianapolis, Indiana 46268, USA)

  • Sunita Gopalan

    (Sangamo BioSciences, Point Richmond Tech Center, 501 Canal Boulevard, Suite A100, Richmond, California 94804, USA)

  • Xiangdong Meng

    (Sangamo BioSciences, Point Richmond Tech Center, 501 Canal Boulevard, Suite A100, Richmond, California 94804, USA)

  • Vivian M. Choi

    (Sangamo BioSciences, Point Richmond Tech Center, 501 Canal Boulevard, Suite A100, Richmond, California 94804, USA)

  • Jeremy M. Rock

    (Sangamo BioSciences, Point Richmond Tech Center, 501 Canal Boulevard, Suite A100, Richmond, California 94804, USA)

  • Ying-Ying Wu

    (Sangamo BioSciences, Point Richmond Tech Center, 501 Canal Boulevard, Suite A100, Richmond, California 94804, USA)

  • George E. Katibah

    (Sangamo BioSciences, Point Richmond Tech Center, 501 Canal Boulevard, Suite A100, Richmond, California 94804, USA)

  • Gao Zhifang

    (Dow AgroSciences, 9330 Zionsville Road, Indianapolis, Indiana 46268, USA)

  • David McCaskill

    (Dow AgroSciences, 9330 Zionsville Road, Indianapolis, Indiana 46268, USA)

  • Matthew A. Simpson

    (Dow AgroSciences, 9330 Zionsville Road, Indianapolis, Indiana 46268, USA)

  • Beth Blakeslee

    (Dow AgroSciences, 9330 Zionsville Road, Indianapolis, Indiana 46268, USA)

  • Scott A. Greenwalt

    (Dow AgroSciences, 9330 Zionsville Road, Indianapolis, Indiana 46268, USA)

  • Holly J. Butler

    (Dow AgroSciences, 9330 Zionsville Road, Indianapolis, Indiana 46268, USA)

  • Sarah J. Hinkley

    (Sangamo BioSciences, Point Richmond Tech Center, 501 Canal Boulevard, Suite A100, Richmond, California 94804, USA)

  • Lei Zhang

    (Sangamo BioSciences, Point Richmond Tech Center, 501 Canal Boulevard, Suite A100, Richmond, California 94804, USA)

  • Edward J. Rebar

    (Sangamo BioSciences, Point Richmond Tech Center, 501 Canal Boulevard, Suite A100, Richmond, California 94804, USA)

  • Philip D. Gregory

    (Sangamo BioSciences, Point Richmond Tech Center, 501 Canal Boulevard, Suite A100, Richmond, California 94804, USA)

  • Fyodor D. Urnov

    (Sangamo BioSciences, Point Richmond Tech Center, 501 Canal Boulevard, Suite A100, Richmond, California 94804, USA)

Abstract

On target for new plant genes The scope for improvement of yield and disease resistance of crop plants by genetic engineering has been limited by the lack of an efficient method for targeted gene modification. Zinc-finger protein technology looks set to fill the gap. This relies on the use of designed zinc-finger nucleases, artificial chimaeric proteins that exploit the natural recognition mechanism of cellular DNA repair machinery, to make sequence-specific double-stranded DNA breaks at a target locus. In this issue two groups report the successful application of this emerging technique. Shukla et al. modify the maize gene IPK1, thereby introducing both herbicide tolerance and modified phytate metabolism into this important crop plant. Townsend et al. target the SuR loci in tobacco plants, conferring resistance to imidazolinone and sulphonylurea herbicides. The method achieves a high frequency of gene targeting and should be suitable for the routine modification of endogenous plant genes.

Suggested Citation

  • Vipula K. Shukla & Yannick Doyon & Jeffrey C. Miller & Russell C. DeKelver & Erica A. Moehle & Sarah E. Worden & Jon C. Mitchell & Nicole L. Arnold & Sunita Gopalan & Xiangdong Meng & Vivian M. Choi &, 2009. "Precise genome modification in the crop species Zea mays using zinc-finger nucleases," Nature, Nature, vol. 459(7245), pages 437-441, May.
    • Handle: RePEc:nat:nature:v:459:y:2009:i:7245:d:10.1038_nature07992
    DOI: 10.1038/nature07992
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    Cited by:

    1. Ulaganathan, Kandasamy & Goud, Sravanthi & Reddy, Madhavi & Kayalvili, Ulaganathan, 2017. "Genome engineering for breaking barriers in lignocellulosic bioethanol production," Renewable and Sustainable Energy Reviews, Elsevier, vol. 74(C), pages 1080-1107.
    2. Joel P. Hague & Stephen L. Dellaporta & Maria A. Moreno & Chip Longo & Kimberly Nelson & Albert P. Kausch, 2012. "Pollen Sterility—A Promising Approach to Gene Confinement and Breeding for Genetically Modified Bioenergy Crops," Agriculture, MDPI, vol. 2(4), pages 1-21, October.

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