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High-frequency modification of plant genes using engineered zinc-finger nucleases

Author

Listed:
  • Jeffrey A. Townsend

    (Development & Cell Biology, Iowa State University, Ames, Iowa 50011, USA)

  • David A. Wright

    (Development & Cell Biology, Iowa State University, Ames, Iowa 50011, USA)

  • Ronnie J. Winfrey

    (Development & Cell Biology, Iowa State University, Ames, Iowa 50011, USA)

  • Fengli Fu

    (Development & Cell Biology, Iowa State University, Ames, Iowa 50011, USA)

  • Morgan L. Maeder

    (Molecular Pathology Unit and Center for Cancer Research, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA
    Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA)

  • J. Keith Joung

    (Molecular Pathology Unit and Center for Cancer Research, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA
    Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
    Harvard Medical School, Boston, Massachusetts 02115, USA)

  • Daniel F. Voytas

    (Cell Biology & Development
    Center for Genome Engineering, University of Minnesota, Minneapolis, Minnesota 55455, USA)

Abstract

On target for new plant genes The scope for improvement of yield and disease resistance of crop plants by genetic engineering has been limited by the lack of an efficient method for targeted gene modification. Zinc-finger protein technology looks set to fill the gap. This relies on the use of designed zinc-finger nucleases, artificial chimaeric proteins that exploit the natural recognition mechanism of cellular DNA repair machinery, to make sequence-specific double-stranded DNA breaks at a target locus. In this issue two groups report the successful application of this emerging technique. Shukla et al. modify the maize gene IPK1, thereby introducing both herbicide tolerance and modified phytate metabolism into this important crop plant. Townsend et al. target the SuR loci in tobacco plants, conferring resistance to imidazolinone and sulphonylurea herbicides. The method achieves a high frequency of gene targeting and should be suitable for the routine modification of endogenous plant genes.

Suggested Citation

  • Jeffrey A. Townsend & David A. Wright & Ronnie J. Winfrey & Fengli Fu & Morgan L. Maeder & J. Keith Joung & Daniel F. Voytas, 2009. "High-frequency modification of plant genes using engineered zinc-finger nucleases," Nature, Nature, vol. 459(7245), pages 442-445, May.
    • Handle: RePEc:nat:nature:v:459:y:2009:i:7245:d:10.1038_nature07845
    DOI: 10.1038/nature07845
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    Cited by:

    1. David VLK & Jana ŘEPKOVÁ, 2017. "Application of next-generation sequencing in plant breeding," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 53(3), pages 89-96.
    2. Joel P. Hague & Stephen L. Dellaporta & Maria A. Moreno & Chip Longo & Kimberly Nelson & Albert P. Kausch, 2012. "Pollen Sterility—A Promising Approach to Gene Confinement and Breeding for Genetically Modified Bioenergy Crops," Agriculture, MDPI, vol. 2(4), pages 1-21, October.

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