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Virus-free induction of pluripotency and subsequent excision of reprogramming factors

Author

Listed:
  • Keisuke Kaji

    (MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, University of Edinburgh)

  • Katherine Norrby

    (MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, University of Edinburgh)

  • Agnieszka Paca

    (MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, University of Edinburgh)

  • Maria Mileikovsky

    (Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada)

  • Paria Mohseni

    (Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada
    University of Toronto, Toronto, Ontario M5S 1A8, Canada)

  • Knut Woltjen

    (Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada)

Abstract

Virus-free iPS cells The discovery that non-germline adult cells can be reprogrammed to become pluripotent, able to differentiate into any cell type, opened up exciting possibilities. Reprogrammed cells — called induced pluripotent stem (iPS) cells — should have great potential in regenerative medicine, but most current methods of producing them involve viral gene delivery that could cause abnormalities in the induced cells. Two groups in this issue report on a collaboration that has succeeded in producing pluripotency in human cells without using viral vectors. Stable iPS cells were produced in both human and mouse fibroblasts using virus-derived 2A peptide sequences to create a multicistronic vector incorporating the reprogramming factors, delivered to the cell by the piggyBac transposon vector. The 2A-linked reprogramming factors, not required in the established iPS cell lines, were then removed.

Suggested Citation

  • Keisuke Kaji & Katherine Norrby & Agnieszka Paca & Maria Mileikovsky & Paria Mohseni & Knut Woltjen, 2009. "Virus-free induction of pluripotency and subsequent excision of reprogramming factors," Nature, Nature, vol. 458(7239), pages 771-775, April.
    • Handle: RePEc:nat:nature:v:458:y:2009:i:7239:d:10.1038_nature07864
    DOI: 10.1038/nature07864
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    Cited by:

    1. Daniel F. Kaemena & Masahito Yoshihara & Meryam Beniazza & James Ashmore & Suling Zhao & Mårten Bertenstam & Victor Olariu & Shintaro Katayama & Keisuke Okita & Simon R. Tomlinson & Kosuke Yusa & Keis, 2023. "B1 SINE-binding ZFP266 impedes mouse iPSC generation through suppression of chromatin opening mediated by reprogramming factors," Nature Communications, Nature, vol. 14(1), pages 1-16, December.

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