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Crystal structure of human spliceosomal U1 snRNP at 5.5 Å resolution

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  • Daniel A. Pomeranz Krummel

    (MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    Present addresses: Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02454-9110, USA (D.A.P.K.); Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, USA (A.K.W.L.).)

  • Chris Oubridge

    (MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK)

  • Adelaine K. W. Leung

    (MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    Present addresses: Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02454-9110, USA (D.A.P.K.); Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, USA (A.K.W.L.).)

  • Jade Li

    (MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK)

  • Kiyoshi Nagai

    (MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK)

Abstract

Human spliceosomal U1 small nuclear ribonucleoprotein particles (snRNPs), which consist of U1 small nuclear RNA and ten proteins, recognize the 5′ splice site within precursor messenger RNAs and initiate the assembly of the spliceosome for intron excision. An electron density map of the functional core of U1 snRNP at 5.5 Å resolution has enabled us to build the RNA and, in conjunction with site-specific labelling of individual proteins, to place the seven Sm proteins, U1-C and U1-70K into the map. Here we present the detailed structure of a spliceosomal snRNP, revealing a hierarchical network of intricate interactions between subunits. A striking feature is the amino (N)-terminal polypeptide of U1-70K, which extends over a distance of 180 Å from its RNA binding domain, wraps around the core domain consisting of the seven Sm proteins and finally contacts U1-C, which is crucial for 5′-splice-site recognition. The structure of U1 snRNP provides insights into U1 snRNP assembly and suggests a possible mechanism of 5′-splice-site recognition.

Suggested Citation

  • Daniel A. Pomeranz Krummel & Chris Oubridge & Adelaine K. W. Leung & Jade Li & Kiyoshi Nagai, 2009. "Crystal structure of human spliceosomal U1 snRNP at 5.5 Å resolution," Nature, Nature, vol. 458(7237), pages 475-480, March.
    • Handle: RePEc:nat:nature:v:458:y:2009:i:7237:d:10.1038_nature07851
    DOI: 10.1038/nature07851
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    Cited by:

    1. Josef Pánek & Adriana Roithová & Nenad Radivojević & Michal Sýkora & Archana Bairavasundaram Prusty & Nicholas Huston & Han Wan & Anna Marie Pyle & Utz Fischer & David Staněk, 2023. "The SMN complex drives structural changes in human snRNAs to enable snRNP assembly," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    2. Sébastien Campagne & Daniel Jutzi & Florian Malard & Maja Matoga & Ksenija Romane & Miki Feldmuller & Martino Colombo & Marc-David Ruepp & Frédéric H-T. Allain, 2023. "Molecular basis of RNA-binding and autoregulation by the cancer-associated splicing factor RBM39," Nature Communications, Nature, vol. 14(1), pages 1-18, December.

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