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Quality control by the ribosome following peptide bond formation

Author

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  • Hani S. Zaher

    (Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA)

  • Rachel Green

    (Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA)

Abstract

The overall fidelity of protein synthesis has been thought to rely on the combined accuracy of two basic processes: the aminoacylation of transfer RNAs with their cognate amino acid by the aminoacyl-tRNA synthetases, and the selection of cognate aminoacyl-tRNAs by the ribosome in cooperation with the GTPase elongation factor EF-Tu. These two processes, which together ensure the specific acceptance of a correctly charged cognate tRNA into the aminoacyl (A) site, operate before peptide bond formation. Here we report the identification of an additional mechanism that contributes to high fidelity protein synthesis after peptidyl transfer, using a well-defined in vitro bacterial translation system. In this retrospective quality control step, the incorporation of an amino acid from a non-cognate tRNA into the growing polypeptide chain leads to a general loss of specificity in the A site of the ribosome, and thus to a propagation of errors that results in abortive termination of protein synthesis.

Suggested Citation

  • Hani S. Zaher & Rachel Green, 2009. "Quality control by the ribosome following peptide bond formation," Nature, Nature, vol. 457(7226), pages 161-166, January.
    • Handle: RePEc:nat:nature:v:457:y:2009:i:7226:d:10.1038_nature07582
    DOI: 10.1038/nature07582
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    Cited by:

    1. Asuteka Nagao & Yui Nakanishi & Yutaro Yamaguchi & Yoshifumi Mishina & Minami Karoji & Takafumi Toya & Tomoya Fujita & Shintaro Iwasaki & Kenjyo Miyauchi & Yuriko Sakaguchi & Tsutomu Suzuki, 2023. "Quality control of protein synthesis in the early elongation stage," Nature Communications, Nature, vol. 14(1), pages 1-20, December.
    2. Jeremy Monroe & Daniel E. Eyler & Lili Mitchell & Indrajit Deb & Abigail Bojanowski & Pooja Srinivas & Christine M. Dunham & Bijoyita Roy & Aaron T. Frank & Kristin S. Koutmou, 2024. "N1-Methylpseudouridine and pseudouridine modifications modulate mRNA decoding during translation," Nature Communications, Nature, vol. 15(1), pages 1-11, December.

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