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Rapid cloning of high-affinity human monoclonal antibodies against influenza virus

Author

Listed:
  • Jens Wrammert

    (Emory University School of Medicine, Atlanta, Georgia 30322, USA)

  • Kenneth Smith

    (Immunobiology and Cancer Research Program, The Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma 73104, USA)

  • Joe Miller

    (Emory University School of Medicine, Atlanta, Georgia 30322, USA)

  • William A. Langley

    (Emory University School of Medicine, Atlanta, Georgia 30322, USA)

  • Kenneth Kokko

    (Emory University School of Medicine, Atlanta, Georgia 30322, USA)

  • Christian Larsen

    (Emory University School of Medicine, Atlanta, Georgia 30322, USA)

  • Nai-Ying Zheng

    (Immunobiology and Cancer Research Program, The Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma 73104, USA)

  • Israel Mays

    (Immunobiology and Cancer Research Program, The Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma 73104, USA)

  • Lori Garman

    (Immunobiology and Cancer Research Program, The Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma 73104, USA)

  • Christina Helms

    (Immunobiology and Cancer Research Program, The Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma 73104, USA)

  • Judith James

    (Arthritis and Immunology Research Program, The Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma 73104, USA
    University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA
    University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA)

  • Gillian M. Air

    (University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA)

  • J. Donald Capra

    (Molecular Immunogenetics Research Program, The Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma 73104, USA
    University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA)

  • Rafi Ahmed

    (Emory University School of Medicine, Atlanta, Georgia 30322, USA)

  • Patrick C. Wilson

    (Immunobiology and Cancer Research Program, The Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma 73104, USA
    University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA
    University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA)

Abstract

Monoclonal antibodies: A production boost The use of antibody therapy has been limited in part because fully human monoclonal antibodies (mAbs) targeting specific antigens are difficult to generate. Now by identifying a time window in which the body's immune response to a particular pathogen is at its peak, Wrammert et al. have developed a new technique for the efficient generation of high-affinity human mAbs. In individuals given a booster vaccination against influenza, the number of influenza specific IgG+ antibody-secreting cells in the bloodstream peaks a week after vaccination, accounting for about 6% of all peripheral B cells. By harvesting B cells during this time window, it was possible to produce more than 50 human mAbs that bound to the three target influenza strains with high affinity. The whole procedure took less than a month, which is much quicker that than conventional methods of producing mAbs. The technique should be applicable to any infectious disease for which vaccines are available.

Suggested Citation

  • Jens Wrammert & Kenneth Smith & Joe Miller & William A. Langley & Kenneth Kokko & Christian Larsen & Nai-Ying Zheng & Israel Mays & Lori Garman & Christina Helms & Judith James & Gillian M. Air & J. D, 2008. "Rapid cloning of high-affinity human monoclonal antibodies against influenza virus," Nature, Nature, vol. 453(7195), pages 667-671, May.
    • Handle: RePEc:nat:nature:v:453:y:2008:i:7195:d:10.1038_nature06890
    DOI: 10.1038/nature06890
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    Cited by:

    1. Kucharski, Adam J. & Gog, Julia R., 2012. "Age profile of immunity to influenza: Effect of original antigenic sin," Theoretical Population Biology, Elsevier, vol. 81(2), pages 102-112.
    2. Justin Lessler & Steven Riley & Jonathan M Read & Shuying Wang & Huachen Zhu & Gavin J D Smith & Yi Guan & Chao Qiang Jiang & Derek A T Cummings, 2012. "Evidence for Antigenic Seniority in Influenza A (H3N2) Antibody Responses in Southern China," PLOS Pathogens, Public Library of Science, vol. 8(7), pages 1-11, July.

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