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Reprogramming of human somatic cells to pluripotency with defined factors

Author

Listed:
  • In-Hyun Park

    (Brigham & Women’s Hospital, Boston, Massachusetts 02115, USA; and Harvard Stem Cell Institute, Cambridge, Massachusetts 02138, USA
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School)

  • Rui Zhao

    (Brigham & Women’s Hospital, Boston, Massachusetts 02115, USA; and Harvard Stem Cell Institute, Cambridge, Massachusetts 02138, USA
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School)

  • Jason A. West

    (Brigham & Women’s Hospital, Boston, Massachusetts 02115, USA; and Harvard Stem Cell Institute, Cambridge, Massachusetts 02138, USA
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School)

  • Akiko Yabuuchi

    (Brigham & Women’s Hospital, Boston, Massachusetts 02115, USA; and Harvard Stem Cell Institute, Cambridge, Massachusetts 02138, USA
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School)

  • Hongguang Huo

    (Brigham & Women’s Hospital, Boston, Massachusetts 02115, USA; and Harvard Stem Cell Institute, Cambridge, Massachusetts 02138, USA
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School)

  • Tan A. Ince

    (Brigham and Women’s Hospital, and)

  • Paul H. Lerou

    (Brigham & Women’s Hospital and Children’s Hospital Boston, Boston, Massachusetts 02115, USA)

  • M. William Lensch

    (Brigham & Women’s Hospital, Boston, Massachusetts 02115, USA; and Harvard Stem Cell Institute, Cambridge, Massachusetts 02138, USA
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School)

  • George Q. Daley

    (Brigham & Women’s Hospital, Boston, Massachusetts 02115, USA; and Harvard Stem Cell Institute, Cambridge, Massachusetts 02138, USA
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School)

Abstract

Pluripotency pertains to the cells of early embryos that can generate all of the tissues in the organism. Embryonic stem cells are embryo-derived cell lines that retain pluripotency and represent invaluable tools for research into the mechanisms of tissue formation. Recently, murine fibroblasts have been reprogrammed directly to pluripotency by ectopic expression of four transcription factors (Oct4, Sox2, Klf4 and Myc) to yield induced pluripotent stem (iPS) cells. Using these same factors, we have derived iPS cells from fetal, neonatal and adult human primary cells, including dermal fibroblasts isolated from a skin biopsy of a healthy research subject. Human iPS cells resemble embryonic stem cells in morphology and gene expression and in the capacity to form teratomas in immune-deficient mice. These data demonstrate that defined factors can reprogramme human cells to pluripotency, and establish a method whereby patient-specific cells might be established in culture.

Suggested Citation

  • In-Hyun Park & Rui Zhao & Jason A. West & Akiko Yabuuchi & Hongguang Huo & Tan A. Ince & Paul H. Lerou & M. William Lensch & George Q. Daley, 2008. "Reprogramming of human somatic cells to pluripotency with defined factors," Nature, Nature, vol. 451(7175), pages 141-146, January.
    • Handle: RePEc:nat:nature:v:451:y:2008:i:7175:d:10.1038_nature06534
    DOI: 10.1038/nature06534
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    Cited by:

    1. María José Pino-Barrio & Elisa García-García & Pablo Menéndez & Alberto Martínez-Serrano, 2015. "V-Myc Immortalizes Human Neural Stem Cells in the Absence of Pluripotency-Associated Traits," PLOS ONE, Public Library of Science, vol. 10(3), pages 1-13, March.

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