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Chromatin remodelling at promoters suppresses antisense transcription

Author

Listed:
  • Iestyn Whitehouse

    (Division of Basic Sciences,)

  • Oliver J. Rando

    (University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA)

  • Jeff Delrow

    (Genomics Resource, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA)

  • Toshio Tsukiyama

    (Division of Basic Sciences,)

Abstract

Chromatin allows the eukaryotic cell to package its DNA efficiently. To understand how chromatin structure is controlled across the Saccharomyces cerevisiae genome, we have investigated the role of the ATP-dependent chromatin remodelling complex Isw2 in positioning nucleosomes. We find that Isw2 functions adjacent to promoter regions where it repositions nucleosomes at the interface between genic and intergenic sequences. Nucleosome repositioning by Isw2 is directional and results in increased nucleosome occupancy of the intergenic region. Loss of Isw2 activity leads to inappropriate transcription, resulting in the generation of both coding and noncoding transcripts. Here we show that Isw2 repositions nucleosomes to enforce directionality on transcription by preventing transcription initiation from cryptic sites. Our analyses reveal how chromatin is organized on a global scale and advance our understanding of how transcription is regulated.

Suggested Citation

  • Iestyn Whitehouse & Oliver J. Rando & Jeff Delrow & Toshio Tsukiyama, 2007. "Chromatin remodelling at promoters suppresses antisense transcription," Nature, Nature, vol. 450(7172), pages 1031-1035, December.
    • Handle: RePEc:nat:nature:v:450:y:2007:i:7172:d:10.1038_nature06391
    DOI: 10.1038/nature06391
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    Cited by:

    1. Colin R Lickwar & Bhargavi Rao & Andrey A Shabalin & Andrew B Nobel & Brian D Strahl & Jason D Lieb, 2009. "The Set2/Rpd3S Pathway Suppresses Cryptic Transcription without Regard to Gene Length or Transcription Frequency," PLOS ONE, Public Library of Science, vol. 4(3), pages 1-7, March.
    2. Wolfram Möbius & Ulrich Gerland, 2010. "Quantitative Test of the Barrier Nucleosome Model for Statistical Positioning of Nucleosomes Up- and Downstream of Transcription Start Sites," PLOS Computational Biology, Public Library of Science, vol. 6(8), pages 1-11, August.

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