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Legionella pneumophila proteins that regulate Rab1 membrane cycling

Author

Listed:
  • Alyssa Ingmundson

    (Section of Microbial Pathogenesis, Yale University School of Medicine, Boyer Center for Molecular Medicine, 295 Congress Avenue, New Haven, Connecticut 06536, USA)

  • Anna Delprato

    (UMASS Medical School Two Biotech, 373 Plantation Street, Worcester, Massachusetts 01605, USA)

  • David G. Lambright

    (UMASS Medical School Two Biotech, 373 Plantation Street, Worcester, Massachusetts 01605, USA)

  • Craig R. Roy

    (Section of Microbial Pathogenesis, Yale University School of Medicine, Boyer Center for Molecular Medicine, 295 Congress Avenue, New Haven, Connecticut 06536, USA)

Abstract

Rab1 is a GTPase that regulates the transport of endoplasmic-reticulum-derived vesicles in eukaryotic cells. The intracellular pathogen Legionella pneumophila subverts Rab1 function to create a vacuole that supports bacterial replication by a mechanism that is not well understood. Here we describe L. pneumophila proteins that control Rab1 activity directly. We show that a region in the DrrA (defect in Rab1 recruitment A) protein required for recruitment of Rab1 to membranes functions as a guanine nucleotide dissociation inhibitor displacement factor. A second region of the DrrA protein stimulated Rab1 activation by functioning as a guanine nucleotide exchange factor. The LepB protein was found to inactivate Rab1 by stimulating GTP hydrolysis, indicating that LepB has GTPase-activating protein activity that regulates removal of Rab proteins from membranes. Thus, L. pneumophila encodes proteins that regulate three distinct biochemical reactions critical for Rab GTPase membrane cycling to redirect Rab1 to the pathogen-occupied vacuole and to control Rab1 function.

Suggested Citation

  • Alyssa Ingmundson & Anna Delprato & David G. Lambright & Craig R. Roy, 2007. "Legionella pneumophila proteins that regulate Rab1 membrane cycling," Nature, Nature, vol. 450(7168), pages 365-369, November.
    • Handle: RePEc:nat:nature:v:450:y:2007:i:7168:d:10.1038_nature06336
    DOI: 10.1038/nature06336
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    Cited by:

    1. Marietta S. Kaspers & Vivian Pogenberg & Christian Pett & Stefan Ernst & Felix Ecker & Philipp Ochtrop & Michael Groll & Christian Hedberg & Aymelt Itzen, 2023. "Dephosphocholination by Legionella effector Lem3 functions through remodelling of the switch II region of Rab1b," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    2. Tobias Sahr & Pedro Escoll & Christophe Rusniok & Sheryl Bui & Gérard Pehau-Arnaudet & Gregory Lavieu & Carmen Buchrieser, 2022. "Translocated Legionella pneumophila small RNAs mimic eukaryotic microRNAs targeting the host immune response," Nature Communications, Nature, vol. 13(1), pages 1-18, December.

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