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Developmental reprogramming after chromosome transfer into mitotic mouse zygotes

Author

Listed:
  • Dieter Egli

    (The Stowers Medical Institute, Harvard University, Cambridge, Massachusetts 02138, USA)

  • Jacqueline Rosains

    (The Stowers Medical Institute, Harvard University, Cambridge, Massachusetts 02138, USA)

  • Garrett Birkhoff

    (The Stowers Medical Institute, Harvard University, Cambridge, Massachusetts 02138, USA)

  • Kevin Eggan

    (The Stowers Medical Institute, Harvard University, Cambridge, Massachusetts 02138, USA)

Abstract

Until now, animal cloning and the production of embryonic stem cell lines by somatic cell nuclear transfer have relied on introducing nuclei into meiotic oocytes. In contrast, attempts at somatic cell nuclear transfer into fertilized interphase zygotes have failed. As a result, it has generally been assumed that unfertilized human oocytes will be required for the generation of tailored human embryonic stem cell lines from patients by somatic cell nuclear transfer. Here we report, however, that, unlike interphase zygotes, mouse zygotes temporarily arrested in mitosis can support somatic cell reprogramming, the production of embryonic stem cell lines and the full-term development of cloned animals. Thus, human zygotes and perhaps human embryonic blastomeres may be useful supplements to human oocytes for the creation of patient-derived human embryonic stem cells.

Suggested Citation

  • Dieter Egli & Jacqueline Rosains & Garrett Birkhoff & Kevin Eggan, 2007. "Developmental reprogramming after chromosome transfer into mitotic mouse zygotes," Nature, Nature, vol. 447(7145), pages 679-685, June.
    • Handle: RePEc:nat:nature:v:447:y:2007:i:7145:d:10.1038_nature05879
    DOI: 10.1038/nature05879
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    Cited by:

    1. Sang Bae Lee & Luciano Garofano & Aram Ko & Fulvio D’Angelo & Brulinda Frangaj & Danika Sommer & Qiwen Gan & KyeongJin Kim & Timothy Cardozo & Antonio Iavarone & Anna Lasorella, 2022. "Regulated interaction of ID2 with the anaphase-promoting complex links progression through mitosis with reactivation of cell-type-specific transcription," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    2. Tiago C. Luis & Nikolaos Barkas & Joana Carrelha & Alice Giustacchini & Stefania Mazzi & Ruggiero Norfo & Bishan Wu & Affaf Aliouat & Jose A. Guerrero & Alba Rodriguez-Meira & Tiphaine Bouriez-Jones &, 2023. "Perivascular niche cells sense thrombocytopenia and activate hematopoietic stem cells in an IL-1 dependent manner," Nature Communications, Nature, vol. 14(1), pages 1-18, December.

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