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Relating ligand binding to activation gating in CNGA2 channels

Author

Listed:
  • Christoph Biskup

    (Institut für Physiologie II, Friedrich-Schiller-Universität Jena, D 07740 Jena, Germany)

  • Jana Kusch

    (Institut für Physiologie II, Friedrich-Schiller-Universität Jena, D 07740 Jena, Germany)

  • Eckhard Schulz

    (Fachhochschule Schmalkalden, Fachbereich Elektrotechnik, Blechhammer, D 98574 Schmalkalden, Germany)

  • Vasilica Nache

    (Institut für Physiologie II, Friedrich-Schiller-Universität Jena, D 07740 Jena, Germany)

  • Frank Schwede

    (BIOLOG Life Science Institute, Flughafendamm 9A, D 28199 Bremen, Germany)

  • Frank Lehmann

    (Dyomics GmbH, Winzerlaer Str. 2, D 07745 Jena, Germany)

  • Volker Hagen

    (Leibniz-Institut für Molekulare Pharmakologie, Robert-Rössle-Str. 10, D 13125 Berlin, Germany)

  • Klaus Benndorf

    (Institut für Physiologie II, Friedrich-Schiller-Universität Jena, D 07740 Jena, Germany)

Abstract

Cyclic nucleotide-gated (CNG) ion channels mediate sensory signal transduction in photoreceptors and olfactory cells. Structurally, CNG channels are heterotetramers composed of either two or three homologue subunits1,2,3,4. Although it is well established that activation is a cooperative process of these subunits5, it remains unknown whether the cooperativity is generated by the ligand binding, the gating, or both, and how the subunits interact. In this study, the action of homotetrameric olfactory-type CNGA2 channels6 was studied in inside-out membrane patches by simultaneously determining channel activation and ligand binding, using the fluorescent cGMP analogue 8-DY547-cGMP as the ligand. At concentrations of 8-DY547-cGMP

Suggested Citation

  • Christoph Biskup & Jana Kusch & Eckhard Schulz & Vasilica Nache & Frank Schwede & Frank Lehmann & Volker Hagen & Klaus Benndorf, 2007. "Relating ligand binding to activation gating in CNGA2 channels," Nature, Nature, vol. 446(7134), pages 440-443, March.
    • Handle: RePEc:nat:nature:v:446:y:2007:i:7134:d:10.1038_nature05596
    DOI: 10.1038/nature05596
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    Cited by:

    1. Klaus Benndorf & Jana Kusch & Eckhard Schulz, 2012. "Probability Fluxes and Transition Paths in a Markovian Model Describing Complex Subunit Cooperativity in HCN2 Channels," PLOS Computational Biology, Public Library of Science, vol. 8(10), pages 1-10, October.
    2. Vishal R. Patel & Arturo M. Salinas & Darong Qi & Shipra Gupta & David J. Sidote & Marcel P. Goldschen-Ohm, 2021. "Single-molecule imaging with cell-derived nanovesicles reveals early binding dynamics at a cyclic nucleotide-gated ion channel," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
    3. Sabine Hummert & Susanne Thon & Thomas Eick & Ralf Schmauder & Eckhard Schulz & Klaus Benndorf, 2018. "Activation gating in HCN2 channels," PLOS Computational Biology, Public Library of Science, vol. 14(3), pages 1-18, March.
    4. Xiaolong Gao & Philipp A. M. Schmidpeter & Vladimir Berka & Ryan J. Durham & Chen Fan & Vasanthi Jayaraman & Crina M. Nimigean, 2022. "Gating intermediates reveal inhibitory role of the voltage sensor in a cyclic nucleotide-modulated ion channel," Nature Communications, Nature, vol. 13(1), pages 1-12, December.

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