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AB5 subtilase cytotoxin inactivates the endoplasmic reticulum chaperone BiP

Author

Listed:
  • Adrienne W. Paton

    (University of Adelaide)

  • Travis Beddoe

    (Department of Biochemistry and Molecular Biology, Monash University)

  • Cheleste M. Thorpe

    (Tufts-New England Medical Center)

  • James C. Whisstock

    (Department of Biochemistry and Molecular Biology, Monash University)

  • Matthew C. J. Wilce

    (Department of Biochemistry and Molecular Biology, Monash University)

  • Jamie Rossjohn

    (Department of Biochemistry and Molecular Biology, Monash University)

  • Ursula M. Talbot

    (University of Adelaide)

  • James C. Paton

    (University of Adelaide)

Abstract

AB5 toxins are produced by pathogenic bacteria and consist of enzymatic A subunits that corrupt essential eukaryotic cell functions, and pentameric B subunits that mediate uptake into the target cell. AB5 toxins include the Shiga, cholera and pertussis toxins and a recently discovered fourth family, subtilase cytotoxin, which is produced by certain Shiga toxigenic strains of Escherichia coli. Here we show that the extreme cytotoxicity of this toxin for eukaryotic cells is due to a specific single-site cleavage of the essential endoplasmic reticulum chaperone BiP/GRP78. The A subunit is a subtilase-like serine protease; structural studies revealed an unusually deep active-site cleft, which accounts for its exquisite substrate specificity. A single amino-acid substitution in the BiP target site prevented cleavage, and co-expression of this resistant protein protected transfected cells against the toxin. BiP is a master regulator of endoplasmic reticulum function, and its cleavage by subtilase cytotoxin represents a previously unknown trigger for cell death.

Suggested Citation

  • Adrienne W. Paton & Travis Beddoe & Cheleste M. Thorpe & James C. Whisstock & Matthew C. J. Wilce & Jamie Rossjohn & Ursula M. Talbot & James C. Paton, 2006. "AB5 subtilase cytotoxin inactivates the endoplasmic reticulum chaperone BiP," Nature, Nature, vol. 443(7111), pages 548-552, October.
    • Handle: RePEc:nat:nature:v:443:y:2006:i:7111:d:10.1038_nature05124
    DOI: 10.1038/nature05124
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    Cited by:

    1. Eduardo Pinho Melo & Tasuku Konno & Ilaria Farace & Mosab Ali Awadelkareem & Lise R. Skov & Fernando Teodoro & Teresa P. Sancho & Adrienne W. Paton & James C. Paton & Matthew Fares & Pedro M. R. Paulo, 2022. "Stress-induced protein disaggregation in the endoplasmic reticulum catalysed by BiP," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    2. Zhenfeng Song & Pattaraporn Thepsuwan & Woosuk Steve Hur & Mauricio Torres & Shuangcheng Alivia Wu & Xiaoqiong Wei & Nusrat Jahan Tushi & Juncheng Wei & Francesca Ferraresso & Adrienne W. Paton & Jame, 2024. "Regulation of hepatic inclusions and fibrinogen biogenesis by SEL1L-HRD1 ERAD," Nature Communications, Nature, vol. 15(1), pages 1-17, December.

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