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Unravelling the dynamics of RNA degradation by ribonuclease II and its RNA-bound complex

Author

Listed:
  • Carlos Frazão

    (Division of Biological Chemistry)

  • Colin E. McVey

    (Division of Biological Chemistry)

  • Mónica Amblar

    (ITQB–Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Apt. 127)

  • Ana Barbas

    (ITQB–Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Apt. 127)

  • Clemens Vonrhein

    (Global Phasing Limited, Sheraton House, Castle Park)

  • Cecília M. Arraiano

    (ITQB–Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Apt. 127)

  • Maria A. Carrondo

    (Division of Biological Chemistry)

Abstract

Keeping RNA up to scratch RNA degradation by class II RNase family members plays a fundamental role in the maturation, turnover and quality control of RNA. The crystal structure of an RNase II has now been determined for the first time, in both RNA bound and ligand free forms. Surprisingly, the domain structure does not correspond to that predicted by sequence analysis. The molecule has two points of contact with the RNA: one site anchors the RNA, and the other catalyses cleavage. The structural details explain why RNase II acts only on single-stranded RNA, and how it moves along the RNA to processively degrade it.

Suggested Citation

  • Carlos Frazão & Colin E. McVey & Mónica Amblar & Ana Barbas & Clemens Vonrhein & Cecília M. Arraiano & Maria A. Carrondo, 2006. "Unravelling the dynamics of RNA degradation by ribonuclease II and its RNA-bound complex," Nature, Nature, vol. 443(7107), pages 110-114, September.
    • Handle: RePEc:nat:nature:v:443:y:2006:i:7107:d:10.1038_nature05080
    DOI: 10.1038/nature05080
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    Cited by:

    1. Zhiheng Yang & Wenzhe Liu & Lihua Zhao & Dongbao Yin & Jianfei Feng & Lidong Li & Xuefeng Guo, 2023. "Single-exonuclease nanocircuits reveal the RNA degradation dynamics of PNPase and demonstrate potential for RNA sequencing," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

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