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RecA acts in trans to allow replication of damaged DNA by DNA polymerase V

Author

Listed:
  • Katharina Schlacher

    (University of Southern California, University Park)

  • Michael M. Cox

    (University of Wisconsin-Madison)

  • Roger Woodgate

    (National Institute of Child Health and Human Development, National Institutes of Health)

  • Myron F. Goodman

    (University of Southern California, University Park)

Abstract

The DNA polymerase V (pol V) and RecA proteins are essential components of a mutagenic translesion synthesis pathway in Escherichia coli designed to cope with DNA damage. Previously, it has been assumed that RecA binds to the DNA template strand being copied. Here we show, however, that pol-V-catalysed translesion synthesis, in the presence or absence of the β-processivity-clamp, occurs only when RecA nucleoprotein filaments assemble or RecA protomers bind on separate single-stranded (ss)DNA molecules in trans. A 3′-proximal RecA filament end on trans DNA is essential for stimulation; however, synthesis is strengthened by further pol V–RecA interactions occurring elsewhere along a trans nucleoprotein filament. We suggest that trans-stimulation of pol V by RecA bound to ssDNA reflects a distinctive regulatory mechanism of mutation that resolves the paradox of RecA filaments assembled in cis on a damaged template strand obstructing translesion DNA synthesis despite the absolute requirement of RecA for SOS mutagenesis.

Suggested Citation

  • Katharina Schlacher & Michael M. Cox & Roger Woodgate & Myron F. Goodman, 2006. "RecA acts in trans to allow replication of damaged DNA by DNA polymerase V," Nature, Nature, vol. 442(7105), pages 883-887, August.
    • Handle: RePEc:nat:nature:v:442:y:2006:i:7105:d:10.1038_nature05042
    DOI: 10.1038/nature05042
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    Cited by:

    1. Andrew Robinson & John P McDonald & Victor E A Caldas & Meghna Patel & Elizabeth A Wood & Christiaan M Punter & Harshad Ghodke & Michael M Cox & Roger Woodgate & Myron F Goodman & Antoine M van Oijen, 2015. "Regulation of Mutagenic DNA Polymerase V Activation in Space and Time," PLOS Genetics, Public Library of Science, vol. 11(8), pages 1-30, August.

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