IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v441y2006i7095d10.1038_nature04817.html
   My bibliography  Save this article

Distribution and three-dimensional structure of AIDS virus envelope spikes

Author

Listed:
  • Ping Zhu

    (Florida State University)

  • Jun Liu

    (Florida State University)

  • Julian Bess

    (AIDS Vaccine Program, SAIC Frederick Inc, National Cancer Institute at Frederick)

  • Elena Chertova

    (AIDS Vaccine Program, SAIC Frederick Inc, National Cancer Institute at Frederick)

  • Jeffrey D. Lifson

    (AIDS Vaccine Program, SAIC Frederick Inc, National Cancer Institute at Frederick)

  • Henry Grisé

    (Florida State University)

  • Gilad A. Ofek

    (Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health)

  • Kenneth A. Taylor

    (Florida State University)

  • Kenneth H. Roux

    (Florida State University)

Abstract

Envelope glycoprotein (Env) spikes on AIDS retroviruses initiate infection of host cells and are therefore targets for vaccine development. Though crystal structures for partial Env subunits are known, the structure and distribution of native Env spikes on virions is obscure. We applied cryoelectron microscopy tomography to define ultrastructural details of spikes. Virions of wild-type human immunodeficiency virus 1 (HIV-1) and a mutant simian immunodeficiency virus (SIV) had ∼14 and ∼73 spikes per particle, respectively, with some clustering of HIV-1 spikes. Three-dimensional averaging showed that the surface glycoprotein (gp120) ‘head’ of each subunit of the trimeric SIV spike contains a primary mass, with two secondary lobes. The transmembrane glycoprotein ‘stalk’ of each trimer is composed of three independent legs that project obliquely from the trimer head, tripod-like. Reconciling available atomic structures with the three-dimensional whole spike density map yields insights into the orientation of Env spike structural elements and possible structural bases of their functions.

Suggested Citation

  • Ping Zhu & Jun Liu & Julian Bess & Elena Chertova & Jeffrey D. Lifson & Henry Grisé & Gilad A. Ofek & Kenneth A. Taylor & Kenneth H. Roux, 2006. "Distribution and three-dimensional structure of AIDS virus envelope spikes," Nature, Nature, vol. 441(7095), pages 847-852, June.
    • Handle: RePEc:nat:nature:v:441:y:2006:i:7095:d:10.1038_nature04817
    DOI: 10.1038/nature04817
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/nature04817
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/nature04817?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Terrence M Dobrowsky & Brian R Daniels & Robert F Siliciano & Sean X Sun & Denis Wirtz, 2010. "Organization of Cellular Receptors into a Nanoscale Junction during HIV-1 Adhesion," PLOS Computational Biology, Public Library of Science, vol. 6(7), pages 1-14, July.
    2. Kun-Wei Chan & Christina C. Luo & Hong Lu & Xueling Wu & Xiang-Peng Kong, 2021. "A site of vulnerability at V3 crown defined by HIV-1 bNAb M4008_N1," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    3. David A. Spencer & Benjamin S. Goldberg & Shilpi Pandey & Tracy Ordonez & Jérémy Dufloo & Philip Barnette & William F. Sutton & Heidi Henderson & Rebecca Agnor & Lina Gao & Timothée Bruel & Olivier Sc, 2022. "Phagocytosis by an HIV antibody is associated with reduced viremia irrespective of enhanced complement lysis," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    4. Carsten Magnus & Roland R Regoes, 2010. "Estimating the Stoichiometry of HIV Neutralization," PLOS Computational Biology, Public Library of Science, vol. 6(3), pages 1-11, March.
    5. Kemin Tan & Junjian Chen & Yu Kaku & Yi Wang & Luke Donius & Rafiq Ahmad Khan & Xiaolong Li & Hannah Richter & Michael S. Seaman & Thomas Walz & Wonmuk Hwang & Ellis L. Reinherz & Mikyung Kim, 2023. "Inadequate structural constraint on Fab approach rather than paratope elicitation limits HIV-1 MPER vaccine utility," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    6. Shuang Yang & Giorgos Hiotis & Yi Wang & Junjian Chen & Jia-huai Wang & Mikyung Kim & Ellis L. Reinherz & Thomas Walz, 2022. "Dynamic HIV-1 spike motion creates vulnerability for its membrane-bound tripod to antibody attack," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    7. Zhentao Sang & Lu Xu & Renyu Ding & Minjun Wang & Xiaoran Yang & Xitan Li & Bingxin Zhou & Kaijun Gou & Yang Han & Tingting Liu & Xuchun Chen & Ying Cheng & Huazhe Yang & Heran Li, 2023. "Nanoparticles exhibiting virus-mimic surface topology for enhanced oral delivery," Nature Communications, Nature, vol. 14(1), pages 1-19, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:441:y:2006:i:7095:d:10.1038_nature04817. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.