IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v440y2006i7087d10.1038_nature04607.html
   My bibliography  Save this article

Designed divergent evolution of enzyme function

Author

Listed:
  • Yasuo Yoshikuni

    (UCSF/UCB Joint Graduate Group in Bioengineering
    Lawrence Berkeley National Laboratory)

  • Thomas E. Ferrin

    (UCSF/UCB Joint Graduate Group in Bioengineering
    University of California at San Francisco)

  • Jay D. Keasling

    (UCSF/UCB Joint Graduate Group in Bioengineering
    Department of Chemical Engineering
    University of California at Berkeley
    Lawrence Berkeley National Laboratory)

Abstract

Enzymes to order Natural molecular evolution has created enzymes designed to catalyse a single reaction, by modifying enzymes that were once much less choosy. Yoshikuni et al. have developed a method of designing synthetic enzymes using a mathematical model that mimics the mechanisms of molecular evolution. They use the system to produce a series of novel sesquiterpene cyclases that use different reaction pathways to produce different end-products. This demonstrates the feasibility of exploiting the evolvability of an enzyme scaffold to produce more pure versions of existing molecules or to create new molecules that do not exist in nature.

Suggested Citation

  • Yasuo Yoshikuni & Thomas E. Ferrin & Jay D. Keasling, 2006. "Designed divergent evolution of enzyme function," Nature, Nature, vol. 440(7087), pages 1078-1082, April.
    • Handle: RePEc:nat:nature:v:440:y:2006:i:7087:d:10.1038_nature04607
    DOI: 10.1038/nature04607
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/nature04607
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/nature04607?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Boxue Tian & C Dale Poulter & Matthew P Jacobson, 2016. "Defining the Product Chemical Space of Monoterpenoid Synthases," PLOS Computational Biology, Public Library of Science, vol. 12(8), pages 1-13, August.
    2. Codruta Ignea & Morten H. Raadam & Aikaterini Koutsaviti & Yong Zhao & Yao-Tao Duan & Maria Harizani & Karel Miettinen & Panagiota Georgantea & Mads Rosenfeldt & Sara E. Viejo-Ledesma & Mikael A. Pete, 2022. "Expanding the terpene biosynthetic code with non-canonical 16 carbon atom building blocks," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    3. Janani Durairaj & Elena Melillo & Harro J Bouwmeester & Jules Beekwilder & Dick de Ridder & Aalt D J van Dijk, 2021. "Integrating structure-based machine learning and co-evolution to investigate specificity in plant sesquiterpene synthases," PLOS Computational Biology, Public Library of Science, vol. 17(3), pages 1-21, March.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:440:y:2006:i:7087:d:10.1038_nature04607. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.