IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v439y2006i7079d10.1038_nature04496.html
   My bibliography  Save this article

Purification and unique properties of mammary epithelial stem cells

Author

Listed:
  • John Stingl

    (British Columbia Cancer Agency
    StemCell Technologies Inc.)

  • Peter Eirew

    (British Columbia Cancer Agency)

  • Ian Ricketson

    (British Columbia Cancer Agency)

  • Mark Shackleton

    (WEHI Biotechnology Centre)

  • François Vaillant

    (WEHI Biotechnology Centre)

  • David Choi

    (British Columbia Cancer Agency)

  • Haiyan I. Li

    (British Columbia Cancer Agency)

  • Connie J. Eaves

    (British Columbia Cancer Agency
    University of British Columbia)

Abstract

Elucidation of the cellular and molecular mechanisms that maintain mammary epithelial tissue integrity is of broad interest and paramount to the design of more effective treatments for breast cancer1. Evidence from both in vitro and in vivo experiments suggests that mammary cell differentiation is a hierarchical process originating in an uncommitted stem cell with self-renewal potential2,3,4. However, analysis of the properties and regulation of mammary stem cells has been limited by a lack of methods for their prospective isolation. Here we report the use of multi-parameter cell sorting and limiting dilution transplant analysis to demonstrate the purification of a rare subset of adult mouse mammary cells that are able individually to regenerate an entire mammary gland within 6 weeks in vivo while simultaneously executing up to ten symmetrical self-renewal divisions. These mammary stem cells are phenotypically distinct from and give rise to mammary epithelial progenitor cells that produce adherent colonies in vitro. The mammary stem cells are also a rapidly cycling population in the normal adult and have molecular features indicative of a basal position in the mammary epithelium.

Suggested Citation

  • John Stingl & Peter Eirew & Ian Ricketson & Mark Shackleton & François Vaillant & David Choi & Haiyan I. Li & Connie J. Eaves, 2006. "Purification and unique properties of mammary epithelial stem cells," Nature, Nature, vol. 439(7079), pages 993-997, February.
    • Handle: RePEc:nat:nature:v:439:y:2006:i:7079:d:10.1038_nature04496
    DOI: 10.1038/nature04496
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/nature04496
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/nature04496?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Antoine A. Khalil & Daan Smits & Peter D. Haughton & Thijs Koorman & Karin A. Jansen & Mathijs P. Verhagen & Mirjam Net & Kitty Zwieten & Lotte Enserink & Lisa Jansen & Abdelrahman G. El-Gammal & Daan, 2024. "A YAP-centered mechanotransduction loop drives collective breast cancer cell invasion," Nature Communications, Nature, vol. 15(1), pages 1-17, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:439:y:2006:i:7079:d:10.1038_nature04496. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.