IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v436y2005i7050d10.1038_nature04010.html
   My bibliography  Save this article

Somatic misexpression of germline P granules and enhanced RNA interference in retinoblastoma pathway mutants

Author

Listed:
  • Duo Wang

    (Harvard Medical School)

  • Scott Kennedy

    (Harvard Medical School
    University of Wisconsin)

  • Darryl Conte

    (Program in Molecular Medicine)

  • John K. Kim

    (Harvard Medical School)

  • Harrison W. Gabel

    (Harvard Medical School)

  • Ravi S. Kamath

    (Harvard Medical School)

  • Craig C. Mello

    (Program in Molecular Medicine
    University of Massachusetts Medical School)

  • Gary Ruvkun

    (Harvard Medical School)

Abstract

Caenorhabditis elegans homologues of the retinoblastoma (Rb) tumour suppressor complex specify cell lineage during development1,2. Here we show that mutations in Rb pathway components enhance RNA interference (RNAi) and cause somatic cells to express genes and elaborate perinuclear structures normally limited to germline-specific P granules. Furthermore, particular gene inactivations that disrupt RNAi reverse the cell lineage transformations of Rb pathway mutants. These findings suggest that mutations in Rb pathway components cause cells to revert to patterns of gene expression normally restricted to germ cells. Rb may act by a similar mechanism to transform mammalian cells.

Suggested Citation

  • Duo Wang & Scott Kennedy & Darryl Conte & John K. Kim & Harrison W. Gabel & Ravi S. Kamath & Craig C. Mello & Gary Ruvkun, 2005. "Somatic misexpression of germline P granules and enhanced RNA interference in retinoblastoma pathway mutants," Nature, Nature, vol. 436(7050), pages 593-597, July.
    • Handle: RePEc:nat:nature:v:436:y:2005:i:7050:d:10.1038_nature04010
    DOI: 10.1038/nature04010
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/nature04010
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/nature04010?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Kohei Ohnishi & Takaaki Sokabe & Toru Miura & Makoto Tominaga & Akane Ohta & Atsushi Kuhara, 2024. "G protein-coupled receptor-based thermosensation determines temperature acclimatization of Caenorhabditis elegans," Nature Communications, Nature, vol. 15(1), pages 1-13, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:436:y:2005:i:7050:d:10.1038_nature04010. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.