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Genome-wide analysis of human kinases in clathrin- and caveolae/raft-mediated endocytosis

Author

Listed:
  • Lucas Pelkmans

    (Max Planck Institute of Molecular Cell Biology and Genetics)

  • Eugenio Fava

    (MPI-CBG High-Throughput Technology Development Studio (HT-TDS))

  • Hannes Grabner

    (MPI-CBG High-Throughput Technology Development Studio (HT-TDS))

  • Michael Hannus

    (Cenix Bioscience GmbH)

  • Bianca Habermann

    (Max Planck Institute of Molecular Cell Biology and Genetics
    Scionics Computer Innovation GmbH)

  • Eberhard Krausz

    (MPI-CBG High-Throughput Technology Development Studio (HT-TDS))

  • Marino Zerial

    (Max Planck Institute of Molecular Cell Biology and Genetics)

Abstract

Endocytosis is a key cellular process, encompassing different entry routes and endocytic compartments. To what extent endocytosis is subjected to high-order regulation by the cellular signalling machinery remains unclear. Using high-throughput RNA interference and automated image analysis, we explored the function of human kinases in two principal types of endocytosis: clathrin- and caveolae/raft-mediated endocytosis. We monitored this through infection of vesicular stomatitis virus, simian virus 40 and transferrin trafficking, and also through cell proliferation and apoptosis assays. Here we show that a high number of kinases are involved in endocytosis, and that each endocytic route is regulated by a specific kinase subset. Notably, one group of kinases exerted opposite effects on the two endocytic routes, suggesting coordinate regulation. Our analysis demonstrates that signalling functions such as those controlling cell adhesion, growth and proliferation, are built into the machinery of endocytosis to a much higher degree than previously recognized.

Suggested Citation

  • Lucas Pelkmans & Eugenio Fava & Hannes Grabner & Michael Hannus & Bianca Habermann & Eberhard Krausz & Marino Zerial, 2005. "Genome-wide analysis of human kinases in clathrin- and caveolae/raft-mediated endocytosis," Nature, Nature, vol. 436(7047), pages 78-86, July.
    • Handle: RePEc:nat:nature:v:436:y:2005:i:7047:d:10.1038_nature03571
    DOI: 10.1038/nature03571
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    Cited by:

    1. Hsiu-Hui Tsai & Hsiao-Jung Kao & Ming-Wei Kuo & Chin-Hsien Lin & Chun-Min Chang & Yi-Yin Chen & Hsiao-Huei Chen & Pui-Yan Kwok & Alice L. Yu & John Yu, 2023. "Whole genomic analysis reveals atypical non-homologous off-target large structural variants induced by CRISPR-Cas9-mediated genome editing," Nature Communications, Nature, vol. 14(1), pages 1-9, December.
    2. Catherine A. Doyle & Gregory W. Busey & Wesley H. Iobst & Volker Kiessling & Chloe Renken & Hansa Doppalapudi & Marta E. Stremska & Mohan C. Manjegowda & Mohd Arish & Weiming Wang & Shardul Naphade & , 2024. "Endosomal fusion of pH-dependent enveloped viruses requires ion channel TRPM7," Nature Communications, Nature, vol. 15(1), pages 1-19, December.

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