IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v435y2005i7046d10.1038_nature03650.html
   My bibliography  Save this article

Phosphoinositide phosphatase activity coupled to an intrinsic voltage sensor

Author

Listed:
  • Yoshimichi Murata

    (National Institutes of Natural Sciences
    National Institutes of Natural Sciences)

  • Hirohide Iwasaki

    (National Institutes of Natural Sciences
    National Institutes of Natural Sciences
    The Graduate University for Advanced Studies)

  • Mari Sasaki

    (National Institutes of Natural Sciences
    National Institutes of Natural Sciences
    The Graduate University for Advanced Studies)

  • Kazuo Inaba

    (University of Tsukuba)

  • Yasushi Okamura

    (National Institutes of Natural Sciences
    National Institutes of Natural Sciences
    The Graduate University for Advanced Studies
    National Institute of Advanced Industrial Science and Technology)

Abstract

Changes in membrane potential affect ion channels and transporters, which then alter intracellular chemical conditions. Other signalling pathways coupled to membrane potential have been suggested1,2,3 but their underlying mechanisms are unknown. Here we describe a novel protein from the ascidian Ciona intestinalis that has a transmembrane voltage-sensing domain homologous to the S1–S4 segments of voltage-gated channels and a cytoplasmic domain similar to phosphatase and tensin homologue. This protein, named C. intestinalis voltage-sensor-containing phosphatase (Ci-VSP), displays channel-like ‘gating’ currents and directly translates changes in membrane potential into the turnover of phosphoinositides. The activity of the phosphoinositide phosphatase in Ci-VSP is tuned within a physiological range of membrane potential. Immunocytochemical studies show that Ci-VSP is expressed in Ciona sperm tail membranes, indicating a possible role in sperm function or morphology. Our data demonstrate that voltage sensing can function beyond channel proteins and thus more ubiquitously than previously realized.

Suggested Citation

  • Yoshimichi Murata & Hirohide Iwasaki & Mari Sasaki & Kazuo Inaba & Yasushi Okamura, 2005. "Phosphoinositide phosphatase activity coupled to an intrinsic voltage sensor," Nature, Nature, vol. 435(7046), pages 1239-1243, June.
  • Handle: RePEc:nat:nature:v:435:y:2005:i:7046:d:10.1038_nature03650
    DOI: 10.1038/nature03650
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/nature03650
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.

    File URL: https://libkey.io/10.1038/nature03650?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Chang Zhao & Parker D. Webster & Alexis Angeli & Francesco Tombola, 2023. "Mechanically-primed voltage-gated proton channels from angiosperm plants," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    2. Spencer C. Guo & Rong Shen & Benoît Roux & Aaron R. Dinner, 2024. "Dynamics of activation in the voltage-sensing domain of Ciona intestinalis phosphatase Ci-VSP," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    3. Yawei Yu & Lin Zhang & Baobin Li & Zhu Fu & Stephen G. Brohawn & Ehud Y. Isacoff, 2024. "Coupling sensor to enzyme in the voltage sensing phosphatase," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    4. Woori Ko & Euna Lee & Jung-Eun Kim & Hyun-Ho Lim & Byung-Chang Suh, 2024. "The plasma membrane inner leaflet PI(4,5)P2 is essential for the activation of proton-activated chloride channels," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    5. Takafumi Kawai & Shin Morioka & Haruhiko Miyata & Rizki Tsari Andriani & Sharmin Akter & Gabriel Toma & Tatsuya Nakagawa & Yuki Oyama & Rie Iida-Norita & Junko Sasaki & Masahiko Watanabe & Kenji Sakim, 2024. "The significance of electrical signals in maturing spermatozoa for phosphoinositide regulation through voltage-sensing phosphatase," Nature Communications, Nature, vol. 15(1), pages 1-14, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:435:y:2005:i:7046:d:10.1038_nature03650. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.