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Friction and torque govern the relaxation of DNA supercoils by eukaryotic topoisomerase IB

Author

Listed:
  • Daniel A. Koster

    (Delft University of Technology)

  • Vincent Croquette

    (Ecole Normale Supérieure)

  • Cees Dekker

    (Delft University of Technology)

  • Stewart Shuman

    (Sloan-Kettering Institute)

  • Nynke H. Dekker

    (Delft University of Technology)

Abstract

Torque about DNA The manoeuvres involved in replication and transcription lead to a build-up of twisting or torsional strain in DNA, and the job of relieving the strain falls to the topoisomerases. Type IB topoisomerases (TopIBs) cut one DNA strand, allow it to swivel around its partner, then rejoin the ends. This removes both positive and negative supercoils. Direct monitoring of a single TopIB molecule now shows that, unlike other topoisomerases, it does not remove a fixed number of supercoils. Rather, the number of supercoils removed, via a mechanism involving friction between rotating DNA and the enzyme cavity, depends on the torque stored in the DNA. The introduction of friction as a factor in DNA–topoisomerase interactions may have important implications for DNA–enzyme interactions in general. The cover depicts TopIB in action.

Suggested Citation

  • Daniel A. Koster & Vincent Croquette & Cees Dekker & Stewart Shuman & Nynke H. Dekker, 2005. "Friction and torque govern the relaxation of DNA supercoils by eukaryotic topoisomerase IB," Nature, Nature, vol. 434(7033), pages 671-674, March.
    • Handle: RePEc:nat:nature:v:434:y:2005:i:7033:d:10.1038_nature03395
    DOI: 10.1038/nature03395
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    Cited by:

    1. Pascal Carrivain & Maria Barbi & Jean-Marc Victor, 2014. "In Silico Single-Molecule Manipulation of DNA with Rigid Body Dynamics," PLOS Computational Biology, Public Library of Science, vol. 10(2), pages 1-13, February.

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