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TRPA1 is a candidate for the mechanosensitive transduction channel of vertebrate hair cells

Author

Listed:
  • David P. Corey

    (Harvard Medical School
    Harvard Medical School)

  • Jaime García-Añoveros

    (Northwestern University Institute for Neurosciences)

  • Jeffrey R. Holt

    (University of Virginia School of Medicine)

  • Kelvin Y. Kwan

    (Harvard Medical School
    Harvard Medical School)

  • Shuh-Yow Lin

    (Harvard Medical School
    Massachusetts Institute of Technology)

  • Melissa A. Vollrath

    (Harvard Medical School
    Harvard Medical School)

  • Andrea Amalfitano

    (Duke University Medical Center)

  • Eunice L.-M. Cheung

    (Harvard Medical School)

  • Bruce H. Derfler

    (Harvard Medical School
    Harvard Medical School)

  • Anne Duggan

    (Northwestern University Institute for Neurosciences)

  • Gwénaëlle S. G. Géléoc

    (University of Virginia School of Medicine)

  • Paul A. Gray

    (Harvard Medical School
    Dana-Farber Cancer Institute)

  • Matthew P. Hoffman

    (Matrix and Morphogenesis Unit, CDBRB, NIDCR, NIH)

  • Heidi L. Rehm

    (Harvard-Partners Genome Center)

  • Daniel Tamasauskas

    (Harvard Medical School
    Harvard Medical School)

  • Duan-Sun Zhang

    (Harvard Medical School
    Harvard Medical School)

Abstract

Mechanical deflection of the sensory hair bundles of receptor cells in the inner ear causes ion channels located at the tips of the bundle to open, thereby initiating the perception of sound. Although some protein constituents of the transduction apparatus are known, the mechanically gated transduction channels have not been identified in higher vertebrates. Here, we investigate TRP (transient receptor potential) ion channels as candidates and find one, TRPA1 (also known as ANKTM1), that meets criteria for the transduction channel. The appearance of TRPA1 messenger RNA expression in hair cell epithelia coincides developmentally with the onset of mechanosensitivity. Antibodies to TRPA1 label hair bundles, especially at their tips, and tip labelling disappears when the transduction apparatus is chemically disrupted. Inhibition of TRPA1 protein expression in zebrafish and mouse inner ears inhibits receptor cell function, as assessed with electrical recording and with accumulation of a channel-permeant fluorescent dye. TRPA1 is probably a component of the transduction channel itself.

Suggested Citation

  • David P. Corey & Jaime García-Añoveros & Jeffrey R. Holt & Kelvin Y. Kwan & Shuh-Yow Lin & Melissa A. Vollrath & Andrea Amalfitano & Eunice L.-M. Cheung & Bruce H. Derfler & Anne Duggan & Gwénaëlle S., 2004. "TRPA1 is a candidate for the mechanosensitive transduction channel of vertebrate hair cells," Nature, Nature, vol. 432(7018), pages 723-730, December.
    • Handle: RePEc:nat:nature:v:432:y:2004:i:7018:d:10.1038_nature03066
    DOI: 10.1038/nature03066
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    Cited by:

    1. Marc Duque & Corinne A. Lee-Kubli & Yusuf Tufail & Uri Magaram & Janki Patel & Ahana Chakraborty & Jose Mendoza Lopez & Eric Edsinger & Aditya Vasan & Rani Shiao & Connor Weiss & James Friend & Sreeka, 2022. "Sonogenetic control of mammalian cells using exogenous Transient Receptor Potential A1 channels," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    2. Ryota Nomura, 2013. "Listening to the Ensemble of Story-Telling Performance and Audience Reactions," Academic Journal of Interdisciplinary Studies, Richtmann Publishing Ltd, vol. 2, October.
    3. A. Catalina Vélez-Ortega & Ruben Stepanyan & Stephanie E. Edelmann & Sara Torres-Gallego & Channy Park & Desislava A. Marinkova & Joshua S. Nowacki & Ghanshyam P. Sinha & Gregory I. Frolenkov, 2023. "TRPA1 activation in non-sensory supporting cells contributes to regulation of cochlear sensitivity after acoustic trauma," Nature Communications, Nature, vol. 14(1), pages 1-16, December.

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