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A candidate NAD+ transporter in an intracellular bacterial symbiont related to Chlamydiae

Author

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  • Ilka Haferkamp

    (Pflanzenphysiologie, Universität Kaiserslautern)

  • Stephan Schmitz-Esser

    (Institut für Ökologie und Naturschutz, Universität Wien)

  • Nicole Linka

    (Pflanzenphysiologie, Universität Kaiserslautern)

  • Claude Urbany

    (Pflanzenphysiologie, Universität Kaiserslautern)

  • Astrid Collingro

    (Institut für Ökologie und Naturschutz, Universität Wien)

  • Michael Wagner

    (Institut für Ökologie und Naturschutz, Universität Wien)

  • Matthias Horn

    (Institut für Ökologie und Naturschutz, Universität Wien)

  • H. Ekkehard Neuhaus

    (Pflanzenphysiologie, Universität Kaiserslautern)

Abstract

Bacteria living within eukaryotic cells can be essential for the survival or reproduction of the host1,2 but in other cases are among the most successful pathogens3,4. Environmental Chlamydiae, including strain UWE25, thrive as obligate intracellular symbionts within protozoa; are recently discovered relatives of major bacterial pathogens of humans; and also infect human cells4,5,6,7. Genome analysis of UWE25 predicted that this symbiont is unable to synthesize the universal electron carrier nicotinamide adenine dinucleotide (NAD+)7. Compensation of limited biosynthetic capacity in intracellular bacteria is usually achieved by import of primary metabolites8,9,10,11. Here, we report the identification of a candidate transporter protein from UWE25 that is highly specific for import of NAD+ when synthesized heterologously in Escherichia coli. The discovery of this candidate NAD+/ADP exchanger demonstrates that intact NAD+ molecules can be transported through cytoplasmic membranes. This protein acts together with a newly discovered nucleotide transporter and an ATP/ADP translocase12, and allows UWE25 to exploit its host cell by means of a sophisticated metabolic parasitism.

Suggested Citation

  • Ilka Haferkamp & Stephan Schmitz-Esser & Nicole Linka & Claude Urbany & Astrid Collingro & Michael Wagner & Matthias Horn & H. Ekkehard Neuhaus, 2004. "A candidate NAD+ transporter in an intracellular bacterial symbiont related to Chlamydiae," Nature, Nature, vol. 432(7017), pages 622-625, December.
    • Handle: RePEc:nat:nature:v:432:y:2004:i:7017:d:10.1038_nature03131
    DOI: 10.1038/nature03131
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    Cited by:

    1. Björn Klabunde & André Wesener & Wilhelm Bertrams & Isabell Beinborn & Nicole Paczia & Kristin Surmann & Sascha Blankenburg & Jochen Wilhelm & Javier Serrania & Kèvin Knoops & Eslam M. Elsayed & Katri, 2023. "NAD+ metabolism is a key modulator of bacterial respiratory epithelial infections," Nature Communications, Nature, vol. 14(1), pages 1-16, December.

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