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Intergenic transcription is required to repress the Saccharomyces cerevisiae SER3 gene

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Listed:
  • Joseph A. Martens

    (Harvard Medical School)

  • Lisa Laprade

    (Harvard Medical School)

  • Fred Winston

    (Harvard Medical School)

Abstract

Transcription by RNA polymerase II in Saccharomyces cerevisiae and in humans is widespread, even in genomic regions that do not encode proteins1,2,3,4,5,6. The purpose of such intergenic transcription is largely unknown, although it can be regulatory7,8. We have discovered a role for one case of intergenic transcription by studying the S. cerevisiae SER3 gene. Our previous results demonstrated that transcription of SER3 is tightly repressed during growth in rich medium9. We now show that the regulatory region of this gene is highly transcribed under these conditions and produces a non-protein-coding RNA (SRG1). Expression of the SRG1 RNA is required for repression of SER3. Additional experiments have demonstrated that repression occurs by a transcription-interference mechanism in which SRG1 transcription across the SER3 promoter interferes with the binding of activators. This work identifies a previously unknown class of transcriptional regulatory genes.

Suggested Citation

  • Joseph A. Martens & Lisa Laprade & Fred Winston, 2004. "Intergenic transcription is required to repress the Saccharomyces cerevisiae SER3 gene," Nature, Nature, vol. 429(6991), pages 571-574, June.
    • Handle: RePEc:nat:nature:v:429:y:2004:i:6991:d:10.1038_nature02538
    DOI: 10.1038/nature02538
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    Cited by:

    1. Haiqing Xu & Chuan Li & Chuan Xu & Jianzhi Zhang, 2023. "Chance promoter activities illuminate the origins of eukaryotic intergenic transcriptions," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    2. Kota Hamamoto & Yusuke Umemura & Shiho Makino & Takashi Fukaya, 2023. "Dynamic interplay between non-coding enhancer transcription and gene activity in development," Nature Communications, Nature, vol. 14(1), pages 1-17, December.

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