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Complex between nidogen and laminin fragments reveals a paradigmatic β-propeller interface

Author

Listed:
  • Junichi Takagi

    (The Center for Blood Research
    Harvard Medical School)

  • Yuting Yang

    (Dana-Farber Cancer Institute
    Harvard Medical School)

  • Jin-huan Liu

    (Dana-Farber Cancer Institute
    Harvard Medical School)

  • Jia-huai Wang

    (Dana-Farber Cancer Institute
    Harvard Medical School)

  • Timothy A. Springer

    (The Center for Blood Research
    Harvard Medical School)

Abstract

Basement membranes are fundamental to tissue organization and physiology in all metazoans. The interaction between laminin and nidogen is crucial to the assembly of basement membranes1,2,3,4. The structure of the interacting domains reveals a six-bladed Tyr-Trp-Thr-Asp (YWTD) β-propeller domain in nidogen bound to laminin epidermal-growth-factor-like (LE) modules III3–5 in laminin (LE3–5). Laminin LE module 4 binds to an amphitheatre-shaped surface on the pseudo-6-fold axis of the β-propeller, and LE module 3 binds over its rim. A Phe residue that shutters the water-filled central aperture of the β-propeller, the rigidity of the amphitheatre, and high shape complementarity enable the construction of an evolutionarily conserved binding surface for LE4 of unprecedentedly high affinity for its small size5. Hypermorphic mutations in the Wnt co-receptor LRP5 (refs 6–9) suggest that a similar YWTD β-propeller interface is used to bind ligands that function in developmental pathways. A related interface, but shifted off-centre from the pseudo-6-fold axis and lacking the shutter over the central aperture, is used in the low-density lipoprotein receptor for an intramolecular interaction that is regulated by pH in receptor recycling10.

Suggested Citation

  • Junichi Takagi & Yuting Yang & Jin-huan Liu & Jia-huai Wang & Timothy A. Springer, 2003. "Complex between nidogen and laminin fragments reveals a paradigmatic β-propeller interface," Nature, Nature, vol. 424(6951), pages 969-974, August.
    • Handle: RePEc:nat:nature:v:424:y:2003:i:6951:d:10.1038_nature01873
    DOI: 10.1038/nature01873
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