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Structure of the core domain of human cardiac troponin in the Ca2+-saturated form

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  • Soichi Takeda

    (Laboratory for Structural Biochemistry, RIKEN Harima Institute at SPring-8
    PRESTO, Japan Science and Technology Corporation (JST)
    National Cardiovascular Center Research Institute)

  • Atsuko Yamashita

    (Laboratory for Structural Biochemistry, RIKEN Harima Institute at SPring-8)

  • Kayo Maeda

    (Laboratory for Structural Biochemistry, RIKEN Harima Institute at SPring-8)

  • Yuichiro Maéda

    (Laboratory for Structural Biochemistry, RIKEN Harima Institute at SPring-8)

Abstract

Troponin is essential in Ca2+ regulation of skeletal and cardiac muscle contraction. It consists of three subunits (TnT, TnC and TnI) and, together with tropomyosin, is located on the actin filament. Here we present crystal structures of the core domains (relative molecular mass of 46,000 and 52,000) of human cardiac troponin in the Ca2+-saturated form. Analysis of the four-molecule structures reveals that the core domain is further divided into structurally distinct subdomains that are connected by flexible linkers, making the entire molecule highly flexible. The α-helical coiled-coil formed between TnT and TnI is integrated in a rigid and asymmetric structure (about 80 Å long), the IT arm, which bridges putative tropomyosin-anchoring regions. The structures of the troponin ternary complex imply that Ca2+ binding to the regulatory site of TnC removes the carboxy-terminal portion of TnI from actin, thereby altering the mobility and/or flexibility of troponin and tropomyosin on the actin filament.

Suggested Citation

  • Soichi Takeda & Atsuko Yamashita & Kayo Maeda & Yuichiro Maéda, 2003. "Structure of the core domain of human cardiac troponin in the Ca2+-saturated form," Nature, Nature, vol. 424(6944), pages 35-41, July.
    • Handle: RePEc:nat:nature:v:424:y:2003:i:6944:d:10.1038_nature01780
    DOI: 10.1038/nature01780
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    Cited by:

    1. Emily A. Chapman & David S. Roberts & Timothy N. Tiambeng & Jãán Andrews & Man-Di Wang & Emily A. Reasoner & Jake A. Melby & Brad H. Li & Donguk Kim & Andrew J. Alpert & Song Jin & Ying Ge, 2023. "Structure and dynamics of endogenous cardiac troponin complex in human heart tissue captured by native nanoproteomics," Nature Communications, Nature, vol. 14(1), pages 1-12, December.

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