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Three modes of synaptic vesicular recycling revealed by single-vesicle imaging

Author

Listed:
  • Sunil P. Gandhi

    (University of California
    Howard Hughes Medical Institute, and Molecular Neurobiology Laboratory, The Salk Institute)

  • Charles F. Stevens

    (Howard Hughes Medical Institute, and Molecular Neurobiology Laboratory, The Salk Institute)

Abstract

Synapses recycle their spent vesicles in order to keep up with on-going neurotransmitter release. To investigate vesicle recycling in the small synapses of hippocampal neurons, we have used an optical recording method that permits us to resolve single-vesicle events. Here we show that an exocytic event can terminate with three modes of vesicle retrieval: a fast (400–860 ms) ‘kiss-and-run’ mode that has a selective fusion pore; a slow (8–21 s) ‘compensatory’ mode; and a ‘stranded’ mode of recycling, in which a vesicle is left on the cell surface until a nerve impulse triggers its retrieval. We have also observed that, in response to a nerve impulse, synapses with low release probability primarily use the kiss-and-run mode, whereas high release probability terminals predominantly use the compensatory mode of vesicle retrieval.

Suggested Citation

  • Sunil P. Gandhi & Charles F. Stevens, 2003. "Three modes of synaptic vesicular recycling revealed by single-vesicle imaging," Nature, Nature, vol. 423(6940), pages 607-613, June.
    • Handle: RePEc:nat:nature:v:423:y:2003:i:6940:d:10.1038_nature01677
    DOI: 10.1038/nature01677
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    Cited by:

    1. Shanley F. Longfield & Rachel S. Gormal & Matis Feller & Pierre Parutto & Jürgen Reingruber & Tristan P. Wallis & Merja Joensuu & George J. Augustine & Ramón Martínez-Mármol & David Holcman & Frédéric, 2024. "Synapsin 2a tetramerisation selectively controls the presynaptic nanoscale organisation of reserve synaptic vesicles," Nature Communications, Nature, vol. 15(1), pages 1-18, December.

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