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Functional and spatial segregation of secretory vesicle pools according to vesicle age

Author

Listed:
  • Rory R. Duncan

    (University of Edinburgh)

  • Jennifer Greaves

    (University of Edinburgh)

  • Ulrich K. Wiegand

    (University of Edinburgh)

  • Ioulia Matskevich

    (University of Edinburgh)

  • Georg Bodammer

    (University of Edinburgh
    MicroEmissive Displays Ltd, Scottish Microelectronics Centre)

  • David K. Apps

    (University of Edinburgh)

  • Michael J. Shipston

    (University of Edinburgh)

  • Robert H. Chow

    (Keck USC School of Medicine)

Abstract

Synaptic terminals and neuroendocrine cells are packed with secretory vesicles, only a few of which are docked at the plasma membrane and readily releasable. The remainder are thought to constitute a large cytoplasmic reserve pool awaiting recruitment into the readily releasable pool (RRP) for exocytosis1. How vesicles are prioritized in recruitment is still unknown: the choice could be random, or else the oldest or the newest ones might be favoured. Here we show, using a fluorescent cargo protein2 that changes colour with time3, that vesicles in bovine adrenal chromaffin cells segregate into distinct populations, based on age. Newly assembled vesicles are immobile (morphologically docked) at the plasma membrane shortly after biogenesis, whereas older vesicles are mobile and located deeper in the cell. Different secretagogues selectively release vesicles from the RRP or, surprisingly, selectively from the deeper cytoplasmic pool. Thus, far from being equal, vesicles are segregated functionally and spatially according to age.

Suggested Citation

  • Rory R. Duncan & Jennifer Greaves & Ulrich K. Wiegand & Ioulia Matskevich & Georg Bodammer & David K. Apps & Michael J. Shipston & Robert H. Chow, 2003. "Functional and spatial segregation of secretory vesicle pools according to vesicle age," Nature, Nature, vol. 422(6928), pages 176-180, March.
    • Handle: RePEc:nat:nature:v:422:y:2003:i:6928:d:10.1038_nature01389
    DOI: 10.1038/nature01389
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