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Crystal structure of bacterial multidrug efflux transporter AcrB

Author

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  • Satoshi Murakami

    (Osaka University
    Osaka University
    CREST, Japan Science and Technology Corporation)

  • Ryosuke Nakashima

    (Osaka University)

  • Eiki Yamashita

    (Osaka University)

  • Akihito Yamaguchi

    (Osaka University
    Osaka University
    CREST, Japan Science and Technology Corporation)

Abstract

AcrB is a major multidrug exporter in Escherichia coli. It cooperates with a membrane fusion protein, AcrA, and an outer membrane channel, TolC. We have determined the crystal structure of AcrB at 3.5 Å resolution. Three AcrB protomers are organized as a homotrimer in the shape of a jellyfish. Each protomer is composed of a transmembrane region 50 Å thick and a 70 Å protruding headpiece. The top of the headpiece opens like a funnel, where TolC might directly dock into AcrB. A pore formed by three α-helices connects the funnel with a central cavity located at the bottom of the headpiece. The cavity has three vestibules at the side of the headpiece which lead into the periplasm. In the transmembrane region, each protomer has twelve transmembrane α-helices. The structure implies that substrates translocated from the cell interior through the transmembrane region and from the periplasm through the vestibules are collected in the central cavity and then actively transported through the pore into the TolC tunnel.

Suggested Citation

  • Satoshi Murakami & Ryosuke Nakashima & Eiki Yamashita & Akihito Yamaguchi, 2002. "Crystal structure of bacterial multidrug efflux transporter AcrB," Nature, Nature, vol. 419(6907), pages 587-593, October.
  • Handle: RePEc:nat:nature:v:419:y:2002:i:6907:d:10.1038_nature01050
    DOI: 10.1038/nature01050
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    Cited by:

    1. Alina Ornik-Cha & Julia Wilhelm & Jessica Kobylka & Hanno Sjuts & Attilio V. Vargiu & Giuliano Malloci & Julian Reitz & Anja Seybert & Achilleas S. Frangakis & Klaas M. Pos, 2021. "Structural and functional analysis of the promiscuous AcrB and AdeB efflux pumps suggests different drug binding mechanisms," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    2. Wanqiu Li & Linlin Wang & Bradley M. Wierbowski & Mo Lu & Feitong Dong & Wenchen Liu & Sisi Li & Peiyi Wang & Adrian Salic & Xin Gong, 2021. "Structural insights into proteolytic activation of the human Dispatched1 transporter for Hedgehog morphogen release," Nature Communications, Nature, vol. 12(1), pages 1-11, December.
    3. Jody L. Andersen & Gui-Xin He & Prathusha Kakarla & Ranjana KC & Sanath Kumar & Wazir Singh Lakra & Mun Mun Mukherjee & Indrika Ranaweera & Ugina Shrestha & Thuy Tran & Manuel F. Varela, 2015. "Multidrug Efflux Pumps from Enterobacteriaceae, Vibrio cholerae and Staphylococcus aureus Bacterial Food Pathogens," IJERPH, MDPI, vol. 12(2), pages 1-61, January.

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