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A proteomic view of the Plasmodium falciparum life cycle

Author

Listed:
  • Laurence Florens

    (The Scripps Research Institute, SR-11)

  • Michael P. Washburn

    (Syngenta Research & Technology
    The Institute for Genomic Research)

  • J. Dale Raine

    (Imperial College of Science, Technology & Medicine, Sir Alexander Fleming Building)

  • Robert M. Anthony

    (Naval Medical Research Center, Malaria Program (IDD))

  • Munira Grainger

    (National Institute for Medical Research)

  • J. David Haynes

    (Naval Medical Research Center, Malaria Program (IDD)
    Walter Reed Army Institute of Research)

  • J. Kathleen Moch

    (Naval Medical Research Center, Malaria Program (IDD))

  • Nemone Muster

    (The Scripps Research Institute, SR-11)

  • John B. Sacci

    (Naval Medical Research Center, Malaria Program (IDD)
    University of Maryland School of Medicine)

  • David L. Tabb

    (The Scripps Research Institute, SR-11
    University of Washington)

  • Adam A. Witney

    (Naval Medical Research Center, Malaria Program (IDD)
    St George's Hospital Medical School, Cranmer Terrace)

  • Dirk Wolters

    (Syngenta Research & Technology
    The Institute for Genomic Research
    Ruhr-University Bochum, Institute of Analytical Chemistry)

  • Yimin Wu

    (American Type Culture Collection)

  • Malcolm J. Gardner

    (The Institute for Genomic Research)

  • Anthony A. Holder

    (National Institute for Medical Research)

  • Robert E. Sinden

    (Imperial College of Science, Technology & Medicine, Sir Alexander Fleming Building)

  • John R. Yates

    (The Scripps Research Institute, SR-11
    Syngenta Research & Technology
    The Institute for Genomic Research)

  • Daniel J. Carucci

    (Naval Medical Research Center, Malaria Program (IDD))

Abstract

The completion of the Plasmodium falciparum clone 3D7 genome provides a basis on which to conduct comparative proteomics studies of this human pathogen. Here, we applied a high-throughput proteomics approach to identify new potential drug and vaccine targets and to better understand the biology of this complex protozoan parasite. We characterized four stages of the parasite life cycle (sporozoites, merozoites, trophozoites and gametocytes) by multidimensional protein identification technology. Functional profiling of over 2,400 proteins agreed with the physiology of each stage. Unexpectedly, the antigenically variant proteins of var and rif genes, defined as molecules on the surface of infected erythrocytes, were also largely expressed in sporozoites. The detection of chromosomal clusters encoding co-expressed proteins suggested a potential mechanism for controlling gene expression.

Suggested Citation

  • Laurence Florens & Michael P. Washburn & J. Dale Raine & Robert M. Anthony & Munira Grainger & J. David Haynes & J. Kathleen Moch & Nemone Muster & John B. Sacci & David L. Tabb & Adam A. Witney & Dir, 2002. "A proteomic view of the Plasmodium falciparum life cycle," Nature, Nature, vol. 419(6906), pages 520-526, October.
    • Handle: RePEc:nat:nature:v:419:y:2002:i:6906:d:10.1038_nature01107
    DOI: 10.1038/nature01107
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