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A regulatory cytoplasmic poly(A) polymerase in Caenorhabditis elegans

Author

Listed:
  • Liaoteng Wang

    (University of Wisconsin-Madison)

  • Christian R. Eckmann

    (University of Wisconsin-Madison)

  • Lisa C. Kadyk

    (University of Wisconsin-Madison
    Exelixis, Inc.)

  • Marvin Wickens

    (University of Wisconsin-Madison)

  • Judith Kimble

    (University of Wisconsin-Madison
    University of Wisconsin-Madison)

Abstract

Messenger RNA regulation is a critical mode of controlling gene expression. Regulation of mRNA stability and translation is linked to controls of poly(A) tail length1,2. Poly(A) lengthening can stabilize and translationally activate mRNAs, whereas poly(A) removal can trigger degradation and translational repression. Germline granules (for example, polar granules in flies, P granules in worms) are ribonucleoprotein particles implicated in translational control3. Here we report that the Caenorhabditis elegans gene gld-2, a regulator of mitosis/meiosis decision and other germline events4, encodes the catalytic moiety of a cytoplasmic poly(A) polymerase (PAP) that is associated with P granules in early embryos. Importantly, the GLD-2 protein sequence has diverged substantially from that of conventional eukaryotic PAPs, and lacks a recognizable RRM (RNA recognition motif)-like domain. GLD-2 has little PAP activity on its own, but is stimulated in vitro by GLD-3. GLD-3 is also a developmental regulator, and belongs to the Bicaudal-C family of RNA binding proteins5. We suggest that GLD-2 is the prototype for a class of regulatory cytoplasmic PAPs that are recruited to specific mRNAs by a binding partner, thereby targeting those mRNAs for polyadenylation and increased expression.

Suggested Citation

  • Liaoteng Wang & Christian R. Eckmann & Lisa C. Kadyk & Marvin Wickens & Judith Kimble, 2002. "A regulatory cytoplasmic poly(A) polymerase in Caenorhabditis elegans," Nature, Nature, vol. 419(6904), pages 312-316, September.
    • Handle: RePEc:nat:nature:v:419:y:2002:i:6904:d:10.1038_nature01039
    DOI: 10.1038/nature01039
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    Cited by:

    1. Michał Brouze & Agnieszka Czarnocka-Cieciura & Olga Gewartowska & Monika Kusio-Kobiałka & Kamil Jachacy & Marcin Szpila & Bartosz Tarkowski & Jakub Gruchota & Paweł Krawczyk & Seweryn Mroczek & Ewa Bo, 2024. "TENT5-mediated polyadenylation of mRNAs encoding secreted proteins is essential for gametogenesis in mice," Nature Communications, Nature, vol. 15(1), pages 1-20, December.

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