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Multiply infected spleen cells in HIV patients

Author

Listed:
  • Andreas Jung

    (University of the Saarland)

  • Reinhard Maier

    (University of the Saarland)

  • Jean-Pierre Vartanian

    (Unité de Rétrovirologie Moléculaire, Institut Pasteur)

  • Gennady Bocharov

    (Institute of Numerical Mathematics, Russian Academy of Sciences)

  • Volker Jung

    (University of the Saarland)

  • Ulrike Fischer

    (University of the Saarland)

  • Eckart Meese

    (University of the Saarland)

  • Simon Wain-Hobson

    (Unité de Rétrovirologie Moléculaire, Institut Pasteur)

  • Andreas Meyerhans

    (University of the Saarland)

Abstract

The genome of the human immunodeficiency virus is highly prone to recombination1,2,3, although it is not obvious whether recombinants arise infrequently or whether they are constantly being spawned but escape identification because of the massive and rapid turnover of virus particles4,5. Here we use fluorescence in situ hybridization to estimate the number of proviruses harboured by individual splenocytes from two HIV patients, and determine the extent of recombination by sequencing amplified DNA from these cells. We find an average of three or four proviruses per cell and evidence for huge numbers of recombinants and extensive genetic variation. Although this creates problems for phylogenetic analyses, which ignore recombination effects, the intracellular variation may help to broaden immune recognition.

Suggested Citation

  • Andreas Jung & Reinhard Maier & Jean-Pierre Vartanian & Gennady Bocharov & Volker Jung & Ulrike Fischer & Eckart Meese & Simon Wain-Hobson & Andreas Meyerhans, 2002. "Multiply infected spleen cells in HIV patients," Nature, Nature, vol. 418(6894), pages 144-144, July.
    • Handle: RePEc:nat:nature:v:418:y:2002:i:6894:d:10.1038_418144a
    DOI: 10.1038/418144a
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    Cited by:

    1. Igor Sazonov & Dmitry Grebennikov & Andreas Meyerhans & Gennady Bocharov, 2021. "Markov Chain-Based Stochastic Modelling of HIV-1 Life Cycle in a CD4 T Cell," Mathematics, MDPI, vol. 9(17), pages 1-19, August.
    2. Rouzine, Igor M. & Coffin, John M., 2010. "Multi-site adaptation in the presence of infrequent recombination," Theoretical Population Biology, Elsevier, vol. 77(3), pages 189-204.
    3. Andrea Arias & Pablo López & Raphael Sánchez & Yasuhiro Yamamura & Vanessa Rivera-Amill, 2018. "Sanger and Next Generation Sequencing Approaches to Evaluate HIV-1 Virus in Blood Compartments," IJERPH, MDPI, vol. 15(8), pages 1-10, August.

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