IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v416y2002i6881d10.1038_nature734.html
   My bibliography  Save this article

The Drosophila immune response against Gram-negative bacteria is mediated by a peptidoglycan recognition protein

Author

Listed:
  • Marie Gottar

    (Institut de Biologie Moléculaire et Cellulaire)

  • Vanessa Gobert

    (Institut de Biologie Moléculaire et Cellulaire)

  • Tatiana Michel

    (Institut de Biologie Moléculaire et Cellulaire)

  • Marcia Belvin

    (Exelixis Inc.)

  • Geoffrey Duyk

    (Exelixis Inc.)

  • Jules A. Hoffmann

    (Institut de Biologie Moléculaire et Cellulaire)

  • Dominique Ferrandon

    (Institut de Biologie Moléculaire et Cellulaire)

  • Julien Royet

    (Institut de Biologie Moléculaire et Cellulaire)

Abstract

The antimicrobial defence of Drosophila relies largely on the challenge-induced synthesis of an array of potent antimicrobial peptides by the fat body1,2. The defence against Gram-positive bacteria and natural fungal infections is mediated by the Toll signalling pathway, whereas defence against Gram-negative bacteria is dependent on the Immune deficiency (IMD) pathway3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18. Loss-of-function mutations in either pathway reduce the resistance to corresponding infections3,9. The link between microbial infections and activation of these two pathways has remained elusive. The Toll pathway is activated by Gram-positive bacteria through a circulating Peptidoglycan recognition protein (PGRP-SA)6. PGRPs appear to be highly conserved from insects to mammals, and the Drosophila genome contains 13 members19,20,21,22,23. Here we report a mutation in a gene coding for a putative transmembrane protein, PGRP-LC, which reduces survival to Gram-negative sepsis but has no effect on the response to Gram-positive bacteria or natural fungal infections. By genetic epistasis, we demonstrate that PGRP-LC acts upstream of the imd gene. The data on PGRP-SA with respect to the response to Gram-positive infections, together with the present report, indicate that the PGRP family has a principal role in sensing microbial infections in Drosophila.

Suggested Citation

  • Marie Gottar & Vanessa Gobert & Tatiana Michel & Marcia Belvin & Geoffrey Duyk & Jules A. Hoffmann & Dominique Ferrandon & Julien Royet, 2002. "The Drosophila immune response against Gram-negative bacteria is mediated by a peptidoglycan recognition protein," Nature, Nature, vol. 416(6881), pages 640-644, April.
    • Handle: RePEc:nat:nature:v:416:y:2002:i:6881:d:10.1038_nature734
    DOI: 10.1038/nature734
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/nature734
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/nature734?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Martina Montanari & Gérard Manière & Martine Berthelot-Grosjean & Yves Dusabyinema & Benjamin Gillet & Yaël Grosjean & C. Léopold Kurz & Julien Royet, 2024. "Larval microbiota primes the Drosophila adult gustatory response," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    2. Aya Yanagawa & Claudine Neyen & Bruno Lemaitre & Frédéric Marion-Poll, 2017. "The gram-negative sensing receptor PGRP-LC contributes to grooming induction in Drosophila," PLOS ONE, Public Library of Science, vol. 12(11), pages 1-15, November.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:416:y:2002:i:6881:d:10.1038_nature734. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.