Author
Listed:
- George L. Dale
(University of Oklahoma Health Sciences Center)
- Paul Friese
(University of Oklahoma Health Sciences Center)
- Peter Batar
(University of Oklahoma Health Sciences Center)
- Stephen F. Hamilton
(College of Pharmacy, University of Oklahoma Health Sciences Center)
- Guy L. Reed
(Cardiovascular Biology Laboratory, Harvard School of Public Health)
- Kenneth W. Jackson
(University of Oklahoma Health Sciences Center)
- Kenneth J. Clemetson
(Theodor Kocher Institute, University of Berne)
- Lorenzo Alberio
(Theodor Kocher Institute, University of Berne)
Abstract
Activated platelets bind numerous adhesive and procoagulant proteins by receptor-mediated processes1. Although there is little evidence to suggest that these processes are heterogeneous in platelets, we previously found that platelets co-stimulated with collagen and thrombin express functional α-granule factor V only on a subpopulation of cells2. Here we show that these cells, referred to as ‘COAT-platelets’, bind additional α-granule proteins, including fibrinogen, von Willebrand factor, thrombospondin, fibronectin and α2-antiplasmin. These proteins are all transglutaminase substrates, and inhibitors of transglutaminase prevent the production of COAT-platelets. A synthetic transglutaminase substrate (CP15) also binds to COAT-platelets, and analysis by high performance liquid chromatography/mass spectrometry shows that a product is formed with a relative molecular mass (Mr) equal to CP15 plus 176. Serotonin, an abundant component of platelet-dense granules3, has an Mr of 176, and fibrinogen isolated from COAT-platelets contains covalently linked serotonin. Synthetic bovine serum albumin-(serotonin)6 binds selectively to COAT-platelets and also inhibits the retention of procoagulant proteins on COAT-platelets. These data indicate that COAT-platelets use serotonin conjugation to augment the retention of procoagulant proteins on their cell surface through an as yet unidentified serotonin receptor.
Suggested Citation
George L. Dale & Paul Friese & Peter Batar & Stephen F. Hamilton & Guy L. Reed & Kenneth W. Jackson & Kenneth J. Clemetson & Lorenzo Alberio, 2002.
"Stimulated platelets use serotonin to enhance their retention of procoagulant proteins on the cell surface,"
Nature, Nature, vol. 415(6868), pages 175-179, January.
Handle:
RePEc:nat:nature:v:415:y:2002:i:6868:d:10.1038_415175a
DOI: 10.1038/415175a
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