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Sensitive detection of pathological prion protein by cyclic amplification of protein misfolding

Author

Listed:
  • Gabriela P. Saborio

    (Serono Pharmaceutical Research Institute)

  • Bruno Permanne

    (Serono Pharmaceutical Research Institute)

  • Claudio Soto

    (Serono Pharmaceutical Research Institute)

Abstract

Prions are the infectious agents responsible for transmissible spongiform encephalopathies. The principal component of prions is the glycoprotein PrPSc, which is a conformationally modified isoform of a normal cell-surface protein called PrPC (ref. 1). During the time between infection and the appearance of the clinical symptoms, minute amounts of PrPSc replicate by conversion of host PrPC, generating large amounts of PrPSc aggregates in the brains of diseased individuals. We aimed to reproduce this event in vitro. Here we report a procedure involving cyclic amplification of protein misfolding that allows a rapid conversion of large excess PrPC into a protease-resistant, PrPSc-like form in the presence of minute quantities of PrPSc template. In this procedure, conceptually analogous to polymerase chain reaction cycling, aggregates formed when PrPSc is incubated with PrPC are disrupted by sonication to generate multiple smaller units for the continued formation of new PrPSc. After cyclic amplification more than 97% of the protease-resistant PrP present in the sample corresponds to newly converted protein. The method could be applied to diagnose the presence of currently undetectable prion infectious agent in tissues and biological fluids, and may provide a unique opportunity to determine whether PrPSc replication results in the generation of infectivity in vitro.

Suggested Citation

  • Gabriela P. Saborio & Bruno Permanne & Claudio Soto, 2001. "Sensitive detection of pathological prion protein by cyclic amplification of protein misfolding," Nature, Nature, vol. 411(6839), pages 810-813, June.
    • Handle: RePEc:nat:nature:v:411:y:2001:i:6839:d:10.1038_35081095
    DOI: 10.1038/35081095
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    Cited by:

    1. Katazyna Milto & Akvile Botyriute & Vytautas Smirnovas, 2013. "Amyloid-Like Fibril Elongation Follows Michaelis-Menten Kinetics," PLOS ONE, Public Library of Science, vol. 8(7), pages 1-5, July.
    2. Victoria A Lawson & Brooke Lumicisi & Jeremy Welton & Dorothy Machalek & Katrina Gouramanis & Helen M Klemm & James D Stewart & Colin L Masters & David E Hoke & Steven J Collins & Andrew F Hill, 2010. "Glycosaminoglycan Sulphation Affects the Seeded Misfolding of a Mutant Prion Protein," PLOS ONE, Public Library of Science, vol. 5(8), pages 1-9, August.
    3. Hasier Eraña & Cristina Sampedro-Torres-Quevedo & Jorge M. Charco & Carlos M. Díaz-Domínguez & Francesca Peccati & Maitena San-Juan-Ansoleaga & Enric Vidal & Nuno Gonçalves-Anjo & Miguel A. Pérez-Cast, 2024. "A Protein Misfolding Shaking Amplification-based method for the spontaneous generation of hundreds of bona fide prions," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    4. Lise Lamoureux & Sharon L R Simon & Margot Plews & Viola Ruddat & Simone Brunet & Catherine Graham & Stefanie Czub & J David Knox, 2013. "Urine Proteins Identified by Two-Dimensional Differential Gel Electrophoresis Facilitate the Differential Diagnoses of Scrapie," PLOS ONE, Public Library of Science, vol. 8(5), pages 1-13, May.

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