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Distinct roles of nerve and muscle in postsynaptic differentiation of the neuromuscular synapse

Author

Listed:
  • Weichun Lin

    (The Salk Institute)

  • Robert W. Burgess

    (Washington University Medical School)

  • Bertha Dominguez

    (The Salk Institute)

  • Samuel L. Pfaff

    (The Salk Institute)

  • Joshua R. Sanes

    (Washington University Medical School)

  • Kuo-Fen Lee

    (The Salk Institute)

Abstract

The development of chemical synapses is regulated by interactions between pre- and postsynaptic cells. At the vertebrate skeletal neuromuscular junction, the organization of an acetylcholine receptor (AChR)-rich postsynaptic apparatus has been well studied. Much evidence suggests that the nerve-derived protein agrin activates muscle-specific kinase (MuSK) to cluster AChRs through the synapse-specific cytoplasmic protein rapsyn. But how postsynaptic differentiation is initiated, or why most synapses are restricted to an ‘end-plate band’ in the middle of the muscle remains unknown. Here we have used genetic methods to address these issues. We report that the initial steps in postsynaptic differentiation and formation of an end-plate band require MuSK and rapsyn, but are not dependent on agrin or the presence of motor axons. In contrast, the subsequent stages of synaptic growth and maintenance require nerve-derived agrin, and a second nerve-derived signal that disperses ectopic postsynaptic apparatus.

Suggested Citation

  • Weichun Lin & Robert W. Burgess & Bertha Dominguez & Samuel L. Pfaff & Joshua R. Sanes & Kuo-Fen Lee, 2001. "Distinct roles of nerve and muscle in postsynaptic differentiation of the neuromuscular synapse," Nature, Nature, vol. 410(6832), pages 1057-1064, April.
  • Handle: RePEc:nat:nature:v:410:y:2001:i:6832:d:10.1038_35074025
    DOI: 10.1038/35074025
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    Cited by:

    1. Ina Klockner & Christian Schutt & Theresa Gerhardt & Thomas Boettger & Thomas Braun, 2022. "Control of CRK-RAC1 activity by the miR-1/206/133 miRNA family is essential for neuromuscular junction function," Nature Communications, Nature, vol. 13(1), pages 1-17, December.

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