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CaT1 manifests the pore properties of the calcium-release-activated calcium channel

Author

Listed:
  • Lixia Yue

    (Children's Hospital, Harvard Medical School, Enders 1309)

  • Ji-Bin Peng

    (Brigham and Women's Hospital and Harvard Medical School)

  • Matthias A. Hediger

    (Brigham and Women's Hospital and Harvard Medical School)

  • David E. Clapham

    (Howard Hughes Medical Institute
    Children's Hospital, Harvard Medical School, Enders 1309)

Abstract

The calcium-release-activated Ca2+channel, ICRAC1,2,3, is a highly Ca2+-selective ion channel that is activated on depletion of either intracellular Ca2+ levels or intracellular Ca2+ stores. The unique gating of ICRAC has made it a favourite target of investigation for new signal transduction mechanisms; however, without molecular identification of the channel protein, such studies have been inconclusive. Here we show that the protein CaT1 (ref. 4), which has six membrane-spanning domains, exhibits the unique biophysical properties of ICRAC when expressed in mammalian cells. Like ICRAC, expressed CaT1 protein is Ca2+ selective, activated by a reduction in intracellular Ca2+ concentration, and inactivated by higher intracellular concentrations of Ca2+. The channel is indistinguishable from ICRAC in the following features: sequence of selectivity to divalent cations; an anomalous mole fraction effect; whole-cell current kinetics; block by lanthanum; loss of selectivity in the absence of divalent cations; and single-channel conductance to Na+ in divalent-ion-free conditions. CaT1 is activated by both passive and active depletion of calcium stores. We propose that CaT1 comprises all or part of the ICRAC pore.

Suggested Citation

  • Lixia Yue & Ji-Bin Peng & Matthias A. Hediger & David E. Clapham, 2001. "CaT1 manifests the pore properties of the calcium-release-activated calcium channel," Nature, Nature, vol. 410(6829), pages 705-709, April.
    • Handle: RePEc:nat:nature:v:410:y:2001:i:6829:d:10.1038_35070596
    DOI: 10.1038/35070596
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