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The S-locus receptor kinase is inhibited by thioredoxins and activated by pollen coat proteins

Author

Listed:
  • Didier Cabrillac

    (Reproduction et Développement des Plantes, UMR 5667 CNRS-INRA-ENSL-UCBL Ecole Normale Supérieure de Lyone)

  • J. Mark Cock

    (Reproduction et Développement des Plantes, UMR 5667 CNRS-INRA-ENSL-UCBL Ecole Normale Supérieure de Lyone)

  • Christian Dumas

    (Reproduction et Développement des Plantes, UMR 5667 CNRS-INRA-ENSL-UCBL Ecole Normale Supérieure de Lyone)

  • Thierry Gaude

    (Reproduction et Développement des Plantes, UMR 5667 CNRS-INRA-ENSL-UCBL Ecole Normale Supérieure de Lyone)

Abstract

The self-incompatibility response in Brassica allows recognition and rejection of self-pollen by the stigmatic papillae. The transmembrane S-locus receptor kinase (SRK), a member of the receptor-like kinase superfamily in plants, mediates recognition of self-pollen on the female side1, whereas the S-locus cysteine-rich protein (SCR) is the male component of the self-incompatibility response2. SCR is presumably located in the pollen coat, and is thought to be the SRK ligand2,3. Although many receptor-like kinases have been isolated in plants, the mechanisms of signal transduction mediated by these molecules remain largely unknown. Here we show that SRK is phosphorylated in vivo within one hour of self-pollination. We also show that, in vitro, autophosphorylation of SRK is prevented by the stigma thioredoxin THL1 in the absence of a ligand. This inhibition is released in a haplotype-specific manner by the addition of pollen coat proteins. Our data indicate that SRK is inhibited by thioredoxins and activated by pollen coat proteins.

Suggested Citation

  • Didier Cabrillac & J. Mark Cock & Christian Dumas & Thierry Gaude, 2001. "The S-locus receptor kinase is inhibited by thioredoxins and activated by pollen coat proteins," Nature, Nature, vol. 410(6825), pages 220-223, March.
    • Handle: RePEc:nat:nature:v:410:y:2001:i:6825:d:10.1038_35065626
    DOI: 10.1038/35065626
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    Cited by:

    1. Xingguo ZHANG & Chaozhi MA & Dongmei YIN & Wei ZHU & Changbin GAO & Jianfeng ZHANG & Tingdong FU, 2013. "Characterization of S haplotype in a new self-compatible Brassica rapa cultivar Dahuangyoucai," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 49(4), pages 157-163.

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