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Polo-like kinase 1 phosphorylates cyclin B1 and targets it to the nucleus during prophase

Author

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  • Fumiko Toyoshima-Morimoto

    (Graduate School of Science, Kyoto University, Sakyo-ku
    Graduate School of Biostudies, Kyoto University, Sakyo-ku)

  • Eri Taniguchi

    (Graduate School of Biostudies, Kyoto University, Sakyo-ku)

  • Nobuko Shinya

    (Central Laboratories for Key Technology, Kirin Brewery Company Limited, Kanazawa-ku, Yokohama-shi)

  • Akihiro Iwamatsu

    (Central Laboratories for Key Technology, Kirin Brewery Company Limited, Kanazawa-ku, Yokohama-shi)

  • Eisuke Nishida

    (Graduate School of Science, Kyoto University, Sakyo-ku
    Graduate School of Biostudies, Kyoto University, Sakyo-ku)

Abstract

In vertebrate cells, the nuclear entry of Cdc2–cyclin B1 (MPF1,2,3) during prophase4,5,6 is thought to be essential for the induction and coordination of M-phase events7,8,9,10,11,12. Phosphorylation of cyclin B1 is central to its nuclear translocation8,13,14, but the kinases that are responsible remain unknown. Here we have purified a protein kinase from Xenopus M-phase extracts that phosphorylates a crucial serine residue (S147) in the middle of the nuclear export signal sequence10,13,15 of cyclin B1. We have identified this kinase as Plx1 (ref. 16), a Xenopus homologue of Polo-like kinase (Plk)-1 (refs 17, 18). During cell-cycle progression in HeLa cells, a change in the kinase activity of endogenous Plk1 toward S147 and/or S133 correlates with a kinase activity in the cell extracts. An anti-Plk1 antibody depletes the M-phase extracts of the kinase activity toward S147 and/or S133. An anti-phospho-S147 antibody reacts specifically with cyclin B1 only during G2/M phase. A mutant cyclin B1 in which S133 and S147 are replaced by alanines remains in the cytoplasm, whereas wild-type cyclin B1 accumulates in the nucleus during prophase. Co-expression of constitutively active Plk1 stimulates nuclear entry of cyclin B1. Our results indicate that Plk1 may be involved in targeting MPF to the nucleus during prophase.

Suggested Citation

  • Fumiko Toyoshima-Morimoto & Eri Taniguchi & Nobuko Shinya & Akihiro Iwamatsu & Eisuke Nishida, 2001. "Polo-like kinase 1 phosphorylates cyclin B1 and targets it to the nucleus during prophase," Nature, Nature, vol. 410(6825), pages 215-220, March.
    • Handle: RePEc:nat:nature:v:410:y:2001:i:6825:d:10.1038_35065617
    DOI: 10.1038/35065617
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    1. Christopher B. Nelson & Samuel Rogers & Kaushik Roychoudhury & Yaw Sing Tan & Caroline J. Atkinson & Alexander P. Sobinoff & Christopher G. Tomlinson & Anton Hsu & Robert Lu & Eloise Dray & Michelle H, 2024. "The Eyes Absent family members EYA4 and EYA1 promote PLK1 activation and successful mitosis through tyrosine dephosphorylation," Nature Communications, Nature, vol. 15(1), pages 1-17, December.

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