IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v410y2001i6824d10.1038_35065084.html
   My bibliography  Save this article

Modulation of the neuronal glutamate transporter EAAC1 by the interacting protein GTRAP3-18

Author

Listed:
  • Chien-liang Glenn Lin

    (Johns Hopkins University, Meyer 6-109)

  • Irina Orlov

    (Johns Hopkins University, Meyer 6-109)

  • Alicia M. Ruggiero

    (Johns Hopkins University, Meyer 6-109)

  • Margaret Dykes-Hoberg

    (Johns Hopkins University, Meyer 6-109)

  • Andy Lee

    (Johns Hopkins University, Meyer 6-109)

  • Mandy Jackson

    (Johns Hopkins University, Meyer 6-109)

  • Jeffrey D. Rothstein

    (Johns Hopkins University, Meyer 6-109)

Abstract

Excitatory amino-acid carrier 1 (EAAC1) is a high-affinity Na+-dependent l-glutamate/d, l-aspartate cell-membrane transport protein1. It is expressed in brain as well as several non-nervous tissues. In brain, EAAC1 is the primary neuronal glutamate transporter2,3. It has a polarized distribution in cells and mainly functions perisynaptically to transport glutamate from the extracellular environment2,3,4. In the kidney it is involved in renal acidic amino-acid re-absorption and amino-acid metabolism5,6,7. Here we describe the identification and characterization of an EAAC1-associated protein, GTRAP3-18. Like EAAC1, GTRAP3-18 is expressed in numerous tissues8,9. It localizes to the cell membrane and cytoplasm, and specifically interacts with carboxy-terminal intracellular domain of EAAC1. Increasing the expression of GTRAP3-18 in cells reduces EAAC1-mediated glutamate transport by lowering substrate affinity. The expression of GTRAP3-18 can be upregulated by retinoic acid, which results in a specific reduction of EAAC1-mediated glutamate transport. These studies show that glutamate transport proteins can be regulated potently and that GTRAP can modulate the transport functions ascribed to EAAC1. GTRAP3-18 may be important in regulating the metabolic function of EAAC1.

Suggested Citation

  • Chien-liang Glenn Lin & Irina Orlov & Alicia M. Ruggiero & Margaret Dykes-Hoberg & Andy Lee & Mandy Jackson & Jeffrey D. Rothstein, 2001. "Modulation of the neuronal glutamate transporter EAAC1 by the interacting protein GTRAP3-18," Nature, Nature, vol. 410(6824), pages 84-88, March.
  • Handle: RePEc:nat:nature:v:410:y:2001:i:6824:d:10.1038_35065084
    DOI: 10.1038/35065084
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/35065084
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.

    File URL: https://libkey.io/10.1038/35065084?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Weidong Zhang & Ayako Miura & Md Moin Abu Saleh & Koichiro Shimizu & Yuichiro Mita & Ryota Tanida & Satoshi Hirako & Seiji Shioda & Valery Gmyr & Julie Kerr-Conte & Francois Pattou & Chunhuan Jin & Yo, 2023. "The NERP-4–SNAT2 axis regulates pancreatic β-cell maintenance and function," Nature Communications, Nature, vol. 14(1), pages 1-21, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:410:y:2001:i:6824:d:10.1038_35065084. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.