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A transcription reinitiation intermediate that is stabilized by activator

Author

Listed:
  • Natalya Yudkovsky

    (The Fred Hutchinson Cancer Research Center, and Molecular and Cellular Biology Program, University of Washington)

  • Jeffrey A. Ranish

    (The Fred Hutchinson Cancer Research Center, and Molecular and Cellular Biology Program, University of Washington
    Institute for Systems Biology)

  • Steven Hahn

    (The Fred Hutchinson Cancer Research Center, and Molecular and Cellular Biology Program, University of Washington
    The Howard Hughes Medical Institute)

Abstract

High levels of gene transcription by RNA polymerase II depend on high rates of transcription initiation and reinitiation. Initiation requires recruitment of the complete transcription machinery to a promoter, a process facilitated by activators and chromatin remodelling factors. Reinitiation probably occurs through a different pathway1. After initiation, a subset of the transcription machinery remains at the promoter, forming a platform for assembly of a second transcription complex2,3,4. Here we describe the isolation of a reinitiation intermediate that includes transcription factors TFIID, TFIIA, TFIIH, TFIIE and Mediator. This intermediate can act as a scaffold for formation of a functional reinitiation complex. Formation of this scaffold is dependent on ATP and TFIIH. The scaffold is stabilized in the presence of the activator Gal4–VP16, but not Gal4–AH, suggesting a new role for some activators and Mediator in promoting high levels of transcription.

Suggested Citation

  • Natalya Yudkovsky & Jeffrey A. Ranish & Steven Hahn, 2000. "A transcription reinitiation intermediate that is stabilized by activator," Nature, Nature, vol. 408(6809), pages 225-229, November.
    • Handle: RePEc:nat:nature:v:408:y:2000:i:6809:d:10.1038_35041603
    DOI: 10.1038/35041603
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    Cited by:

    1. Zhong Chen & William Hankey & Yue Zhao & Jeff Groth & Furong Huang & Hongyan Wang & Alexandre Rosa Campos & Jiaoti Huang & Robert G. Roeder & Qianben Wang, 2021. "Transcription recycling assays identify PAF1 as a driver for RNA Pol II recycling," Nature Communications, Nature, vol. 12(1), pages 1-12, December.

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