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Interleukin 21 and its receptor are involved in NK cell expansion and regulation of lymphocyte function

Author

Listed:
  • Julia Parrish-Novak

    (Departments of Functional Cloning)

  • Stacey R. Dillon

    (Departments of Immunology)

  • Andrew Nelson

    (Departments of Immunology)

  • Angie Hammond

    (Departments of Functional Cloning)

  • Cindy Sprecher

    (Departments of Functional Cloning)

  • Jane A. Gross

    (Departments of Immunology)

  • Janet Johnston

    (Departments of Immunology)

  • Karen Madden

    (Departments of Functional Cloning)

  • Wenfeng Xu

    (Departments of Functional Cloning)

  • Jim West

    (Departments of Protein Biochemistry)

  • Sara Schrader

    (Departments of Functional Cloning)

  • Steve Burkhead

    (Departments of Functional Cloning)

  • Mark Heipel

    (Departments of Functional Cloning)

  • Cameron Brandt

    (Departments of Functional Cloning)

  • Joseph L. Kuijper

    (Departments of Functional Cloning)

  • Janet Kramer

    (Departments of Functional Cloning)

  • Darrell Conklin

    (Departments of Biomolecular Informatics)

  • Scott R. Presnell

    (Departments of Biomolecular Informatics)

  • Jon Berry

    (Departments of Protein Biochemistry)

  • Faith Shiota

    (Departments of Immunology)

  • Susan Bort

    (Departments of Immunology)

  • Kevin Hambly

    (Departments of Immunology)

  • Sherri Mudri

    (Departments of Immunology)

  • Chris Clegg

    (Departments of Immunology)

  • Margaret Moore

    (Genetics, ZymoGenetics, Inc.)

  • Francis J. Grant

    (Departments of Biomolecular Informatics)

  • Catherine Lofton-Day

    (Genetics, ZymoGenetics, Inc.)

  • Teresa Gilbert

    (Genetics, ZymoGenetics, Inc.)

  • Fenella Raymond

    (Genetics, ZymoGenetics, Inc.)

  • Andrew Ching

    (Departments of Biomolecular Informatics)

  • Lena Yao

    (Genetics, ZymoGenetics, Inc.)

  • Deb Smith

    (Genetics, ZymoGenetics, Inc.)

  • Philippa Webster

    (Genetics, ZymoGenetics, Inc.)

  • Theodore Whitmore

    (Genetics, ZymoGenetics, Inc.)

  • Mark Maurer

    (Genetics, ZymoGenetics, Inc.)

  • Kenneth Kaushansky

    (University of Washington, School of Medicine)

  • Rick D. Holly

    (Departments of Functional Cloning)

  • Don Foster

    (Departments of Functional Cloning)

Abstract

Cytokines are important in the regulation of haematopoiesis and immune responses, and can influence lymphocyte development. Here we have identified a class I cytokine receptor that is selectively expressed in lymphoid tissues and is capable of signal transduction. The full-length receptor was expressed in BaF3 cells, which created a functional assay for ligand detection and cloning. Conditioned media from activated human CD3+ T cells supported proliferation of the assay cell line. We constructed a complementary DNA expression library from activated human CD3+ T cells, and identified a cytokine with a four-helix-bundle structure using functional cloning. This cytokine is most closely related to IL2 and IL15, and has been designated IL21 with the receptor designated IL21R. In vitro assays suggest that IL21 has a role in the proliferation and maturation of natural killer (NK) cell populations from bone marrow, in the proliferation of mature B-cell populations co-stimulated with anti-CD40, and in the proliferation of T cells co-stimulated with anti-CD3.

Suggested Citation

  • Julia Parrish-Novak & Stacey R. Dillon & Andrew Nelson & Angie Hammond & Cindy Sprecher & Jane A. Gross & Janet Johnston & Karen Madden & Wenfeng Xu & Jim West & Sara Schrader & Steve Burkhead & Mark , 2000. "Interleukin 21 and its receptor are involved in NK cell expansion and regulation of lymphocyte function," Nature, Nature, vol. 408(6808), pages 57-63, November.
    • Handle: RePEc:nat:nature:v:408:y:2000:i:6808:d:10.1038_35040504
    DOI: 10.1038/35040504
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